Neutron activation analysis and X-ray Rayleigh and Raman scattering of hair and nail clippings as noninvasive bioindicators for Cu liver status in Labrador Retrievers

Neutron activation analysis and X-ray Rayleigh and Raman scattering of hair and nail clippings as noninvasive bioindicators for Cu liver status in Labrador Retrievers

Bode, P.
Bueno, M.I.
Bortoleto, G.G.
Hoffmann, G.
Van den Ingh, T.S.
Rothuizen, J.

Applied Sciences

Radiation, Radionuclides and Reactors

2008-02-09

The heritability of chronic hepatitis in the Labrador Retriever is studied with the aim of identifying the related gene mutation. Identification of cases and controls is largely based on instrumental neutron activation analysis (INAA) Cu determination in liver biopsies. The burden for these companion animals may be reduced if nail clippings and hair (fur) could serve as a noninvasive indicator for the hepatic Cu concentrations. No correlation was found between hepatic Cu concentrations and Cu concentrations in hair and nail samples. However, hair and nail samples were also analyzed by X-ray tube excitation, taking advantage of the X-ray Compton, Rayleigh, and Raman scattering which reflects the organic components such as the type of melanin. Principal component analysis provided first indications that some differentiation between healthy and sick dogs could indeed be obtained from hair and nail analysis.

neutron activation analysis
x-ray spectroscopy
chemometrics
trace elements
biological samples

http://resolver.tudelft.nl/uuid:cbffcf30-62f4-4c92-8060-d7cb6818df64

Springer

1618-2642

Analytical and Bioanalytical Chemistry, 390 (6), 2008

Institutional Repository

journal article

(c) 2008 The Author(s) ; Springer