Print Email Facebook Twitter In vitro co-culture models for the assessment of orthopedic antibacterial biomaterials Title In vitro co-culture models for the assessment of orthopedic antibacterial biomaterials Author Eijkel, B.I.M. (TU Delft Biomaterials & Tissue Biomechanics) Apachitei, I. (TU Delft Biomaterials & Tissue Biomechanics) Fratila-Apachitei, E.L. (TU Delft Biomaterials & Tissue Biomechanics) Zadpoor, A.A. (TU Delft Biomaterials & Tissue Biomechanics) Date 2024 Abstract The antibacterial biofunctionality of bone implants is essential for the prevention and treatment of implant-associated infections (IAI). In vitro co-culture models are utilized to assess this and study bacteria-host cell interactions at the implant interface, aiding our understanding of biomaterial and the immune response against IAI without impeding the peri-implant bone tissue regeneration. This paper reviews existing co-culture models together with their characteristics, results, and clinical relevance. A total of 36 studies were found involving in vitro co-culture models between bacteria and osteogenic or immune cells at the interface with orthopedic antibacterial biomaterials. Most studies (∼67%) involved co-culture models of osteogenic cells and bacteria (osteo-bac), while 33% were co-culture models of immune cells and bacterial cells (im-bac). All models involve direct co-culture of two different cell types. The cell seeding sequence (simultaneous, bacteria-first, and cell-first) was used to mimic clinically relevant conditions and showed the greatest effect on the outcome for both types of co-culture models. The im-bac models are considered more relevant for early peri-implant infections, whereas the osteo-bac models suit late infections. The limitations of the current models and future directions to develop more relevant co-culture models to address specific research questions are also discussed. Subject implant-associated infectionsantibacterial biomaterialsin vitro co-culture modelsosteogenic cellsimmune cells To reference this document use: http://resolver.tudelft.nl/uuid:d3295532-eeaf-4c79-b5b6-70011f4253a3 DOI https://doi.org/10.3389/fbioe.2024.1332771 ISSN 2296-4185 Source Frontiers in Bioengineering and Biotechnology, 12 Part of collection Institutional Repository Document type review Rights © 2024 B.I.M. Eijkel, I. Apachitei, E.L. Fratila-Apachitei, A.A. Zadpoor Files PDF fbioe-12-1332771.pdf 1.8 MB Close viewer /islandora/object/uuid:d3295532-eeaf-4c79-b5b6-70011f4253a3/datastream/OBJ/view