Print Email Facebook Twitter Genotoxicity in the absence of inflammation after tungsten inhalation in mice Title Genotoxicity in the absence of inflammation after tungsten inhalation in mice Author Sørli, Jorid B. (National Research Centre for the Working Environment, Copenhagen) Jensen, Alexander C.Ø. (National Research Centre for the Working Environment, Copenhagen) Mortensen, Alicja (National Research Centre for the Working Environment, Copenhagen) Szarek, Józef (University of Warmia and Mazury) Gutierrez, Claudia A.T. (University of Copenhagen; National Research Centre for the Working Environment, Copenhagen) Hafez, Iosif (The Cyprus Insitute) Biskos, G. (TU Delft Atmospheric Remote Sensing; The Cyprus Insitute) Hougaard, Karin S. (University of Copenhagen; National Research Centre for the Working Environment, Copenhagen) Vogel, Ulla (Technical University of Denmark; National Research Centre for the Working Environment, Copenhagen) Date 2023 Abstract Tungsten is used in several applications and human exposure may occur. To assess its pulmonary toxicity, we exposed male mice to nose-only inhalation of tungsten particles at 9, 23 or 132 mg/m3 (Low, Mid and High exposure) (45 min/day, 5 days/week for 2 weeks). Increased genotoxicity (assessed by comet assay) was seen in bronchoalveolar (BAL) fluid cells at Low and High exposure. We measured acellular ROS production, and cannot exclude that ROS contributed to the observed genotoxicity. We saw no effects on body weight gain, pulmonary inflammation, lactate dehydrogenase or protein in BAL fluid, pathology of liver or kidney, or on sperm counts. In conclusion, tungsten showed non-dose dependent genotoxicity in the absence of inflammation and therefore interpreted to be primary genotoxicity. Based on genotoxicity, a Lowest Observed Adverse Effect Concentration (LOAEC) could be set at 9 mg/m3. It was not possible to establish a No Adverse Effect Concentration (NOAEC). Subject Comet assayPulmonaryToxicityToxicologyWolfram To reference this document use: http://resolver.tudelft.nl/uuid:e0c1ac33-6dc0-4193-9be7-a057aaf65d75 DOI https://doi.org/10.1016/j.etap.2023.104074 ISSN 1382-6689 Source Environmental Toxicology and Pharmacology, 98 Part of collection Institutional Repository Document type journal article Rights © 2023 Jorid B. Sørli, Alexander C.Ø. Jensen, Alicja Mortensen, Józef Szarek, Claudia A.T. Gutierrez, Iosif Hafez, G. Biskos, Karin S. Hougaard, Ulla Vogel, More Authors Files PDF 1_s2.0_S1382668923000157_main.pdf 891.49 KB Close viewer /islandora/object/uuid:e0c1ac33-6dc0-4193-9be7-a057aaf65d75/datastream/OBJ/view