Print Email Facebook Twitter A Peroxygenase-Alcohol Dehydrogenase Cascade Reaction to Transform Ethylbenzene Derivatives into Enantioenriched Phenylethanols Title A Peroxygenase-Alcohol Dehydrogenase Cascade Reaction to Transform Ethylbenzene Derivatives into Enantioenriched Phenylethanols Author Xu, X. (TU Delft BT/Biocatalysis) Brasselet, H.L.Y. (TU Delft BT/Biocatalysis) Jongkind, E.P.J. (TU Delft BT/Biocatalysis) Alcalde, Miguel (University of the Balearic Islands) Paul, C.E. (TU Delft BT/Biocatalysis) Hollmann, F. (TU Delft BT/Biocatalysis) Date 2022 Abstract In this study, we developed a new bienzymatic reaction to produce enantioenriched phenylethanols. In a first step, the recombinant, unspecific peroxygenase from Agrocybe aegerita (rAaeUPO) was used to oxidise ethylbenzene and its derivatives to the corresponding ketones (prochiral intermediates) followed by enantioselective reduction into the desired (R)- or (S)-phenylethanols using the (R)-selective alcohol dehydrogenase (ADH) from Lactobacillus kefir (LkADH) or the (S)-selective ADH from Rhodococcus ruber (ADH-A). In a one-pot two-step cascade, 11 ethylbenzene derivatives were converted into the corresponding chiral alcohols at acceptable yields and often excellent enantioselectivity. To reference this document use: http://resolver.tudelft.nl/uuid:f15cc938-6210-42d8-bdcc-d1e7ce22bfd1 DOI https://doi.org/10.1002/cbic.202200017 ISSN 1439-4227 Source ChemBioChem: a European journal of chemical biology, 23 (6) Part of collection Institutional Repository Document type journal article Rights © 2022 X. Xu, H.L.Y. Brasselet, E.P.J. Jongkind, Miguel Alcalde, C.E. Paul, F. Hollmann Files PDF ChemBioChem_2022_Xu_A_Per ... s_into.pdf 1.03 MB Close viewer /islandora/object/uuid:f15cc938-6210-42d8-bdcc-d1e7ce22bfd1/datastream/OBJ/view