Print Email Facebook Twitter Process for creating a double-stranded polyribonucleotide sequence with terminal overhang, as well as a process for creating a double-stranded polynucleotide construct and an application Title Process for creating a double-stranded polyribonucleotide sequence with terminal overhang, as well as a process for creating a double-stranded polynucleotide construct and an application Author Dekker, N.H. Veenhuizen, P. Faculty Applied Sciences Date 2005-12-15 Abstract The invention relates to a process for creating double-stranded RNA (16) having a terminal overhang. In accordance with the invention a DNA amplification is used for this purpose, followed by a transcription of the amplified DNA. When amplifying the DNA, primer pairs are used and care is taken that additional sequences (18, 19) are present that will ultimately provide the terminal overhangs. To reference this document use: http://resolver.tudelft.nl/uuid:f16ef251-80da-4c1c-8351-44886c3b8fe9 Publisher European Patent Office Source WO 2005118807 (A1) Is part of http://v3.espacenet.com/publicationDetails/biblio?adjacent=true&locale=en_EP&FT=D&date=20051215&CC=WO&NR=2005118807A1&KC=A1 Part of collection Institutional Repository Document type patent Rights (c) 2005 Dekker, N.H.; Veenhuizen, P. Files PDF WO2005118807A1.pdf 811.98 KB Close viewer /islandora/object/uuid:f16ef251-80da-4c1c-8351-44886c3b8fe9/datastream/OBJ/view