Print Email Facebook Twitter Assessing Peroxygenase-Mediated Oxidations in the Presence of High Concentrations of Water-Miscible Co-Solvents Title Assessing Peroxygenase-Mediated Oxidations in the Presence of High Concentrations of Water-Miscible Co-Solvents Author Hilberath, T. (TU Delft BT/Biocatalysis) van Troost, A.G.L. (Student TU Delft) Alcalde, Miguel (Institute of Catalysis, CSIC, Madrid) Hollmann, F. (TU Delft BT/Biocatalysis) Date 2022 Abstract The use of water-miscible organic co-solvents in biocatalysis is a simple procedure for obtaining higher enzymatic activities toward hydrophobic substrates. However, effects on activity and stability have to be carefully evaluated, also with regard to the type and concentration of the respective co-solvent. In this contribution, we investigated and evaluated the effect of some common water-miscible co-solvents on the biocatalytic performance of the recombinant unspecific peroxygenase rAaeUPO from Agrocybe aegerita. rAaeUPO showed promising activities in the presence of high concentrations of the best co-solvent acetonitrile, which enabled to use higher substrate concentrations (≥100 mM). Employing high acetonitrile concentrations for UPO-mediated oxidation of ethylbenzene to (R)-1-phenylethanol was demonstrated under preparative scale conditions and led to product accumulation rates of 31 mM h−1. Subject peroxygenaseoxyfunctionalizationco-solventydroxylationpoxidationmedium engineering To reference this document use: http://resolver.tudelft.nl/uuid:f8c6f4bf-db0f-483c-aedd-8c9be76b7c33 DOI https://doi.org/10.3389/fctls.2022.882992 ISSN 2673-7841 Source Frontiers in Catalysis, 2 Part of collection Institutional Repository Document type journal article Rights © 2022 T. Hilberath, A.G.L. van Troost, Miguel Alcalde, F. Hollmann Files PDF fctls_02_882992.pdf 1.39 MB Close viewer /islandora/object/uuid:f8c6f4bf-db0f-483c-aedd-8c9be76b7c33/datastream/OBJ/view