Print Email Facebook Twitter Under pressure: evolutionary engineering of yeast strains for improved performance in fuels and chemicals production Title Under pressure: evolutionary engineering of yeast strains for improved performance in fuels and chemicals production Author Mans, R. (TU Delft BT/Industriele Microbiologie) Daran, J.G. (TU Delft BT/Industriele Microbiologie) Pronk, J.T. (TU Delft BT/Industriele Microbiologie) Date 2018 Abstract Evolutionary engineering, which uses laboratory evolution to select for industrially relevant traits, is a popular strategy in the development of high-performing yeast strains for industrial production of fuels and chemicals. By integrating whole-genome sequencing, bioinformatics, classical genetics and genome-editing techniques, evolutionary engineering has also become a powerful approach for identification and reverse engineering of molecular mechanisms that underlie industrially relevant traits. New techniques enable acceleration of in vivo mutation rates, both across yeast genomes and at specific loci. Recent studies indicate that phenotypic trade-offs, which are often observed after evolution under constant conditions, can be mitigated by using dynamic cultivation regimes. Advances in research on synthetic regulatory circuits offer exciting possibilities to extend the applicability of evolutionary engineering to products of yeasts whose synthesis requires a net input of cellular energy. To reference this document use: http://resolver.tudelft.nl/uuid:19b09f8e-efce-440d-937b-f10aa442f50d DOI https://doi.org/10.1016/j.copbio.2017.10.011 ISSN 0958-1669 Source Current Opinion in Biotechnology, 50, 47-56 Part of collection Institutional Repository Document type review Rights © 2018 R. Mans, J.G. Daran, J.T. Pronk Files PDF 1_s2.0_S0958166917302288_main.pdf 632.39 KB Close viewer /islandora/object/uuid:19b09f8e-efce-440d-937b-f10aa442f50d/datastream/OBJ/view