Print Email Facebook Twitter Accumulation of defense systems in phage-resistant strains of Pseudomonas aeruginosa Title Accumulation of defense systems in phage-resistant strains of Pseudomonas aeruginosa Author Martins Costa, A.R. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) van den Berg, D.F. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) Esser, J.Q. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) Muralidharan, A. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) van den Bossche, Halewijn (Kavli institute of nanoscience Delft) Estrada Bonilla, B.A. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) van der Steen, B.A. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) Haas, Pieter Jan (University Medical Center Utrecht) Brouns, S.J.J. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) Date 2024 Abstract Prokaryotes encode multiple distinct anti-phage defense systems in their genomes. However, the impact of carrying a multitude of defense systems on phage resistance remains unclear, especially in a clinical context. Using a collection of antibiotic-resistant clinical strains of Pseudomonas aeruginosa and a broad panel of phages, we demonstrate that defense systems contribute substantially to defining phage host range and that overall phage resistance scales with the number of defense systems in the bacterial genome. We show that many individual defense systems target specific phage genera and that defense systems with complementary phage specificities co-occur in P. aeruginosa genomes likely to provide benefits in phage-diverse environments. Overall, we show that phage-resistant phenotypes of P. aeruginosa with at least 19 phage defense systems exist in the populations of clinical, antibiotic-resistant P. aeruginosa strains. To reference this document use: http://resolver.tudelft.nl/uuid:48abb16f-c037-4048-b642-8d60e8557d9a DOI https://doi.org/10.1126/sciadv.adj0341 Embargo date 2024-08-23 ISSN 2375-2548 Source Science Advances, 10 (8), eadj0341 Bibliographical note Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public. Part of collection Institutional Repository Document type journal article Rights © 2024 A.R. Martins Costa, D.F. van den Berg, J.Q. Esser, A. Muralidharan, Halewijn van den Bossche, B.A. Estrada Bonilla, B.A. van der Steen, Pieter Jan Haas, S.J.J. Brouns, More Authors Files file embargo until 2024-08-23