Print Email Facebook Twitter In Vivo distribution of hydrocortisone over whole blood: A novel method for the extraction of erythrocytes Title In Vivo distribution of hydrocortisone over whole blood: A novel method for the extraction of erythrocytes Author Driessen, O. Treuren, L. Moolenaar, A. Meijer, J.W.A. Verheijen, P. Faculty Applied Sciences Date 1990-01-01 Abstract Ten mg hydrocortisone (HC) was administered intravenously to a healthy volunteer after a dexamethasone suppresion test and HC concentrations were determined from 1-270 min in plasma, plasma water and on erythrocytes. HC was extracted front erythrocyte concentrates with high efficiency by HC-poor plasma or by human or bovine albyum solutions. Determination of HC in the plasma of the volunteer mainly gave insight about the concentration time course of HC bound to plasma proteins. One minute after HC injection the amount associated with erythrocytes was about half the amount bound to plasma proteins. Decrease of HC in plasma, and hence on plasma proteins, was monophasic front 30-270 rain with a half-life of 116 rain. Decrease of HC associated with erythrocytes and HC free in plasma water was biphasic from 30-270 min and initially HC diminished about five times faster from these compartments than from plasma proteins. At the end of the observation period half lives on plasma proteins, erythrocytes and in plasma water were similar, i.e., @ 120 rain. It is concluded from these as well as from previous in vitro experiments that erythrocytes gain importance as HC carriers at increasing HC blood concentrations. Once charged, erythrocytes yield HC much more readily than do plasma proteins. This "’last come first go ’" phenomenon of association of HC with erythrocytes is known also to exist for certain drugs. It indicates erythrocytes as important transporters of non-freely ht water soluble compounds. Subject hydrocortisoneerythrocyte extractionerythrocyte bindingprotein bindingfree fraction To reference this document use: http://resolver.tudelft.nl/uuid:8efef75e-e959-4a28-bfa5-ceee03a2c52f Publisher Prous Science ISSN 0379-0355 Source Methods and findings in experimental and clinical pharmacology, 12 (2) 1990 Part of collection Institutional Repository Document type journal article Rights (c) 1990 Driessen, O., Treuren, L., Moolenaar, A., Meijer, J.W.A., Verheijen, P. Files PDF Driessen1990_Valproate.pdf 614.13 KB Close viewer /islandora/object/uuid:8efef75e-e959-4a28-bfa5-ceee03a2c52f/datastream/OBJ/view