Print Email Facebook Twitter Continuous Fatty Acid Decarboxylation using an Immobilized Photodecarboxylase in a Membrane Reactor Title Continuous Fatty Acid Decarboxylation using an Immobilized Photodecarboxylase in a Membrane Reactor Author Zhou, Jianle (South China University of Technology) Hollmann, F. (TU Delft BT/Biocatalysis) He, Qi (South China University of Technology) Chen, Wen (South China University of Technology) Ma, Yunjian (South China University of Technology) Wang, Yonghua (South China University of Technology; GuangdongYoumei Institute of Intelligent Bio-manufacturing Co. Ltd., v) Date 2023 Abstract The realm of photobiocatalytic alkane biofuel synthesis has burgeoned recently; however, the current dearth of well-established and scalable production methodologies in this domain remains conspicuous. In this investigation, we engineered a modified form of membrane-associated fatty acid photodecarboxylase sourced from Micractinium conductrix (McFAP). This endeavour resulted in creating an innovative assembled photoenzyme-membrane (protein load 5 mg cm−2), subsequently integrated into an illuminated flow apparatus to achieve uninterrupted generation of alkane biofuels. Through batch experiments, the photoenzyme-membrane exhibited its prowess in converting fatty acids spanning varying chain lengths (C6–C18). Following this, the membrane-flow mesoscale reactor attained a maximum space-time yield of 1.2 mmol L−1 h−1 (C8) and demonstrated commendable catalytic proficiency across eight consecutive cycles, culminating in a cumulative runtime of eight hours. These findings collectively underscored the photoenzyme-membrane's capability to facilitate the biotransformation of diverse fatty acids, furnishing valuable benchmarks for the conversion of biomass via photobiocatalysis. Subject alkane biofuelcontinuous photocatalysisdecarboxylationphotoenzyme-membraneself-assembly To reference this document use: http://resolver.tudelft.nl/uuid:90f1dd5c-15b1-48f2-924e-bb7b86c422f8 DOI https://doi.org/10.1002/cssc.202301326 Embargo date 2024-06-15 ISSN 1864-5631 Source ChemSusChem (Print): chemistry & sustainability, energy & materials, 17 (3) Bibliographical note Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public. Part of collection Institutional Repository Document type journal article Rights © 2023 Jianle Zhou, F. Hollmann, Qi He, Wen Chen, Yunjian Ma, Yonghua Wang Files PDF ChemSusChem_2023_Zhou_Con ... ane_1_.pdf 2.77 MB Close viewer /islandora/object/uuid:90f1dd5c-15b1-48f2-924e-bb7b86c422f8/datastream/OBJ/view