Print Email Facebook Twitter Structural biology of microbial gas vesicles Title Structural biology of microbial gas vesicles: historical milestones and current knowledge Author Huber, S. (TU Delft BN/Arjen Jakobi Lab) Jakobi, A. (TU Delft BN/Arjen Jakobi Lab) Date 2024 Abstract Gas vesicles mediate buoyancy-based motility in aquatic bacteria and archaea and are the only protein-based structures known to enclose a gas-filled volume. Their unique physicochemical properties and ingenious architecture rank them among the most intriguing macromolecular assemblies characterised to date. This review covers the 60-year journey in quest for a high-resolution structural model of gas vesicles, first highlighting significant strides made in establishing the detailed ultrastructure of gas vesicles through transmission electron microscopy, X-ray fibre diffraction, atomic force microscopy, and NMR spectroscopy. We then survey the recent progress in cryogenic electron microscopy studies of gas vesicles, which eventually led to a comprehensive atomic model of the mature assembly. Synthesising insight from these structures, we examine possible mechanisms of gas vesicle biogenesis and growth, presenting a testable model to guide future experimental work. We conclude by discussing future directions in the structural biology of gas vesicles, particularly considering advancements in AI-driven structure prediction. To reference this document use: http://resolver.tudelft.nl/uuid:96fc37eb-854e-4427-870a-86ef71575f64 DOI https://doi.org/10.1042/bst20230396 ISSN 0300-5127 Source Biochemical Society Transactions, 2024, 205-215 Part of collection Institutional Repository Document type review Rights © 2024 S. Huber, A. Jakobi Files PDF bst-2023-0396c.pdf 1.48 MB Close viewer /islandora/object/uuid:96fc37eb-854e-4427-870a-86ef71575f64/datastream/OBJ/view