Print Email Facebook Twitter Nanopore electro-osmotic trap for the label-free study of single proteins and their conformations Title Nanopore electro-osmotic trap for the label-free study of single proteins and their conformations Author Schmid, S. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft; Wageningen University & Research) Stömmer, Pierre (Technische Universität München) Dietz, Hendrik (Technische Universität München) Dekker, C. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Date 2021 Abstract Many strategies have been pursued to trap and monitor single proteins over time to detect the molecular mechanisms of these essential nanomachines. Single-protein sensing with nanopores is particularly attractive because it allows label-free high-bandwidth detection on the basis of ion currents. Here we present the nanopore electro-osmotic trap (NEOtrap) that allows trapping and observing single proteins for hours with submillisecond time resolution. The NEOtrap is formed by docking a DNA-origami sphere onto a passivated solid-state nanopore, which seals off a nanocavity of a user-defined size and creates an electro-osmotic flow that traps nearby particles irrespective of their charge. We demonstrate the NEOtrap’s ability to sensitively distinguish proteins on the basis of size and shape, and discriminate between nucleotide-dependent protein conformations, as exemplified by the chaperone protein Hsp90. Given the experimental simplicity and capacity for label-free single-protein detection over the broad bio-relevant time range, the NEOtrap opens new avenues to study the molecular kinetics underlying protein function. To reference this document use: http://resolver.tudelft.nl/uuid:ea6e16b7-b1f9-40c9-bafe-a974bdc7de37 DOI https://doi.org/10.1038/s41565-021-00958-5 Embargo date 2022-03-01 ISSN 1748-3387 Source Nature Nanotechnology, 16 (11), 1244-1250 Bibliographical note Accepted Author Manuscript Part of collection Institutional Repository Document type journal article Rights © 2021 S. Schmid, Pierre Stömmer, Hendrik Dietz, C. Dekker Files PDF 41565_2021_958_OnlinePDF.pdf 2.22 MB Close viewer /islandora/object/uuid:ea6e16b7-b1f9-40c9-bafe-a974bdc7de37/datastream/OBJ/view