Pages
- 1
- 2
- document
-
den Ridder, M.J. (author)Mass spectrometry-based cellular proteomics has taken a prominent role in many fields of research, including life sciences, biotechnology and microbial ecology. Still, advanced mass spectrometry-based proteomics methods are not routinely applied to microbes. The cell factory and model organism Saccharomyces cerevisiae, for example, is very well...doctoral thesis 2023
- document
-
den Ridder, M.J. (author), van den Brandeler, Wiebeke (author), Altiner, Meryem (author), Daran-Lapujade, P.A.S. (author), Pabst, Martin (author)The yeast Saccharomyces cerevisiae is a widely-used eukaryotic model organism and a promising cell factory for industry. However, despite decades of research, the regulation of its metabolism is not yet fully understood, and its complexity represents a major challenge for engineering and optimizing biosynthetic routes. Recent studies have...journal article 2023
- document
-
Malubhoy, Zahabiya (author), Bahia, Frederico Mendonça (author), de Valk, Sophie Claire (author), de Hulster, A.F. (author), Rendulić, Toni (author), Ortiz, Juan Paulo Ragas (author), Xiberras, Joeline (author), Klein, Mathias (author), Mans, R. (author), Nevoigt, Elke (author)Background: The microbial production of succinic acid (SA) from renewable carbon sources via the reverse TCA (rTCA) pathway is a process potentially accompanied by net-fixation of carbon dioxide (CO<sub>2</sub>). Among reduced carbon sources, glycerol is particularly attractive since it allows a nearly twofold higher CO<sub>2</sub>-fixation...journal article 2022
- document
-
Genetic bases for the metabolism of the DMS precursor S-methylmethionine by Saccharomyces cerevisiaeEder, Matthias (author), Sanchez, Isabelle (author), Camarasa, Carole (author), Daran, J.G. (author), Legras, Jean Luc (author), Dequin, Sylvie (author)Dimethyl sulfide (DMS) is a sulfur containing volatile that enhances general fruity aroma and imparts aromatic notes in wine. The most important precursor of DMS is S-methylmethionine (SMM), which is synthesized by grapes and can be metabolized by the yeast S. cerevisiae during wine fermentation. Precursor molecules left after fermentation...journal article 2022
- document
-
den Ridder, M.J. (author), Knibbe, E. (author), van den Brandeler, Wiebeke (author), Daran-Lapujade, P.A.S. (author), Pabst, Martin (author)The importance of obtaining comprehensive and accurate information from cellular proteomics experiments asks for a systematic investigation of sample preparation protocols. In particular when working with unicellular organisms with strong cell walls, such as found in the model organism and cell factory Saccharomyces cerevisiae. Here, we...journal article 2022
- document
-
van Aalst, A.C.A. (author), Mans, R. (author), Pronk, J.T. (author)Background: Saccharomyces cerevisiae is intensively used for industrial ethanol production. Its native fermentation pathway enables a maximum product yield of 2 mol of ethanol per mole of glucose. Based on conservation laws, supply of additional electrons could support even higher ethanol yields. However, this option is disallowed by the...journal article 2022
- document
-
Bouwknegt, J. (author), Wiersma, S.J. (author), Ortiz Merino, R.A. (author), Doornenbal, E.S.R. (author), Buitenhuis, Petrik (author), Giera, Martin (author), Müller, Christoph (author), Pronk, J.T. (author)Biosynthesis of sterols, which are key constituents of canonical eukaryotic membranes, requiresmolecular oxygen. Anaerobic protists and deep-branching anaerobic fungi are the only eukaryotes in which a mechanism for sterol-independent growth has been elucidated. In these organisms, tetrahymanol, formed through oxygen-independent cyclization...journal article 2021
- document
-
Bouwknegt, J. (author), Koster, C.C. (author), Vos, A.M. (author), Ortiz Merino, R.A. (author), Wassink, Mats (author), Luttik, M.A.H. (author), van den Broek, M.A. (author), Hagedoorn, P.L. (author), Pronk, J.T. (author)Background: In most fungi, quinone-dependent Class-II dihydroorotate dehydrogenases (DHODs) are essential for pyrimidine biosynthesis. Coupling of these Class-II DHODHs to mitochondrial respiration makes their in vivo activity dependent on oxygen availability. Saccharomyces cerevisiae and closely related yeast species harbor a cytosolic Class...journal article 2021
- document
-
D'Ambrosio, Vasil (author), Dore, Eleonora (author), Di Blasi, Roberto (author), van den Broek, M.A. (author), Sudarsan, Suresh (author), Horst, Jolanda ter (author), Ambri, Francesca (author), Keasling, Jay D. (author), Mans, R. (author)Engineering living cells for production of chemicals, enzymes and therapeutics can burden cells due to use of limited native co-factor availability and/or expression burdens, totalling a fitness deficit compared to parental cells encoded through long evolutionary trajectories to maximise fitness. Ultimately, this discrepancy puts a selective...journal article 2020
- document
-
Gorter de Vries, A.R. (author)Lager beer, also referred to as Pilsner, is the most popular alcoholic beverage in the world, with an annual consumption of almost 200 billion litres per year. To make lager beer, brewer’s wort is fermented with the yeast <i style="mso-bidi-font-style:normal"...doctoral thesis 2019
- document
-
Rajkumar, Arun S. (author), Varela, Javier A. (author), Jürgens, H. (author), Daran, J.G. (author), Morrissey, John P. (author)Kluyveromyces marxianus is a non-conventional yeast whose physiology and metabolism lends itself to diverse biotechnological applications. While the wild-type yeast is already in use for producing fragrances and fermented products, the lack of standardised tools for its genetic and metabolic engineering prevent it from being used as a next...journal article 2019
- document
-
Verhoeven, M.D. (author)The recent start-up of several full-scale ‘second generation’ ethanol plants marks a major milestone in the development of Saccharomyces cerevisiae yeast strains for fermentation of lignocellulosic hydrolysates of agricultural residues and energy crops. In contrast to the fermentation of hexose sugar-rich substrates, such as corn syrup or sugar...doctoral thesis 2018
- document
-
Papapetridis, I. (author), Goudriaan, M. (author), Vazquez Vitali, M. (author), De Keijzer, Nikita A. (author), van den Broek, M.A. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)Background: Reduction or elimination of by-product formation is of immediate economic relevance in fermentation processes for industrial bioethanol production with the yeast Saccharomyces cerevisiae. Anaerobic cultures of wild-type S. cerevisiae require formation of glycerol to maintain the intracellular NADH/NAD<sup>+</sup> balance....journal article 2018
- document
-
Bracher, J.M. (author), Verhoeven, M.D. (author), Wisselink, H. Wouter (author), Crimi, B. (author), Nijland, Jeroen G. (author), Driessen, Arnold J.M. (author), Klaassen, Paul (author), van Maris, A.J.A. (author), Daran, J.G. (author), Pronk, J.T. (author)Background: l-Arabinose occurs at economically relevant levels in lignocellulosic hydrolysates. Its low-affinity uptake via the Saccharomyces cerevisiae Gal2 galactose transporter is inhibited by d-glucose. Especially at low concentrations of l-arabinose, uptake is an important rate-controlling step in the complete conversion of these...journal article 2018
- document
-
Brickwedde, A. (author), Brouwers, N. (author), van den Broek, M.A. (author), Gallego Murillo, Joan Sebastián (author), Fraiture, J.L. (author), Pronk, J.T. (author), Daran, J.G. (author)Saccharomyces pastorianus lager brewing yeasts are domesticated hybrids of Saccharomyces cerevisiae and cold-tolerant Saccharomyces eubayanus. To understand the contribution of both parental genomes to maltose metabolism in brewing wort, this study focuses on maltose transport in the S. eubayanus type strain CBS 12357<sup>T</sup>/FM1318. To...journal article 2018
- document
-
Verhoeven, M.D. (author), de Valk, S.C. (author), Daran, J.G. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)D-Glucose, D-xylose and L-arabinose are major sugars in lignocellulosic hydrolysates. This study explores fermentation of glucose-xylose-arabinose mixtures by a consortium of three ‘specialist’ Saccharomyces cerevisiae strains. A D-glucose- and L-arabinose-tolerant xylose specialist was constructed by eliminating hexose phosphorylation in an...journal article 2018
- document
-
Suarez Mendez, C.A. (author), Ras, C. (author), Wahl, S.A. (author)Background: Natural and industrial environments are dynamic with respect to substrate availability and other conditions like temperature and pH. Especially, metabolism is strongly affected by changes in the extracellular space. Here we study the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast...journal article 2017
- document
-
Papapetridis, I. (author), van Dijk, M. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)Background: Glycerol, whose formation contributes to cellular redox balancing and osmoregulation in Saccharomyces cerevisiae, is an important by-product of yeast-based bioethanol production. Replacing the glycerol pathway by an engineered pathway for NAD<sup>+</sup>-dependent acetate reduction has been shown to improve ethanol yields and...journal article 2017
- document
-
Gonzalez Ramos, D. (author), Gorter de Vries, A.R. (author), Grijseels, Sietske S. (author), van Berkum, M.C. (author), Swinnen, Steve (author), van den Broek, M.A. (author), Nevoigt, Elke (author), Daran, J.G. (author), Pronk, J.T. (author), van Maris, A.J.A. (author)Background: Acetic acid, released during hydrolysis of lignocellulosic feedstocks for second generation bioethanol production, inhibits yeast growth and alcoholic fermentation. Yeast biomass generated in a propagation step that precedes ethanol production should therefore express a high and constitutive level of acetic acid tolerance before...journal article 2016
- document
-
Vos, T. (author), Hakkaart, X.D.V. (author), de Hulster, A.F. (author), van Maris, A.J.A. (author), Pronk, J.T. (author), Daran-Lapujade, P.A.S. (author)Background: Saccharomyces cerevisiae is an established microbial platform for production of native and non-native compounds. When product pathways compete with growth for precursors and energy, uncoupling of growth and product formation could increase product yields and decrease formation of biomass as a by-product. Studying non-growing,...journal article 2016
Pages
- 1
- 2