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Qiao, Li (author), Zhang, Jing (author), Jiang, Yongjian (author), Ma, Bianqin (author), Chen, Haomin (author), Gao, Peng (author), Zhang, Pengfei (author), Wang, Anming (author), Sheldon, R.A. (author)Effective photolytic regeneration of the NAD(P)H cofactor in enzymatic reductions is an important and elusive goal in biocatalysis. It can, in principle, be achieved using a near-infrared light (NIR) driven artificial photosynthesis system employing H<sub>2</sub>O as the sacrificial reductant. To this end we utilized TiO<sub>2</sub>/reduced...journal article 2024
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Luo, Zhiyuan (author), Qiao, Li (author), Chen, Haomin (author), Mao, Zhili (author), Wu, Shujiao (author), Ma, Bianqin (author), Xie, Tian (author), Wang, Anming (author), Pei, Xiaolin (author), Sheldon, R.A. (author)The design and orderly layered co-immobilization of multiple enzymes on resin particles remain challenging. In this study, the SpyTag/SpyCatcher binding pair was fused to the N-terminus of an alcohol dehydrogenase (ADH) and an aldo-keto reductase (AKR), respectively. A non-canonical amino acid (ncAA), p-azido-L-phenylalanine (p-AzF), as the...journal article 2024