Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency

Understanding the impact of guide RNA (gRNA) and genomic locus on CRISPR-Cas9 activity is crucial to design effective gene editing assays. However, it is challenging to profile Cas9 activity in the endogenous cellular environment. Here we leverage our TRIP technology to integrate ~ 1k barcoded reporter genes in the genomes of mouse embryonic...

Standardizing the Dutch Climate Adaptation Stress Test

Due to increasing climate change and its impact on our environment, an increasing amount of policies and scientific research are focused on climate risk reduction. Recent developments in international and Dutch national policies focused on assessing, evaluating and reducing climate risks by spatial adaptation. The assessment of this risk is...

The Future of Water Research: Supporting the implementation of citizen science data collection by investigating the current water quality and quantity situation in the main water sources of the Kathmandu Valley

Kathmandu is a prime example of a city exhibiting both rapid metropolitan region expansion and a population boom. This growth leads to a water stress and pollution of the surface and groundwater. The lack of proper waste management and sanitation results in the ongoing deterioration of the water quality in the Kathmandu Valley. The situation is...

Large variety in a panel of human colon cancer organoids in response to EZH2 inhibition

EZH2 inhibitors have gained great interest for their use as anti-cancer therapeutics. However, most research has focused on EZH2 mutant cancers and recently adverse effects of EZH2 inactivation have come to light. To determine whether colorectal cancer cells respond to EZH2 inhibition and to explore which factors influence the degree of response...

Deep water winch using synthetic fiber rope

Parallel Natural Language Parsing: From Analysis to Speedup

Het optimaliseren van de cascobouw

Manieren tot verbeteren van de cascobouw

Industrierobots in station 1 bij GNM, een modelsimulatie

Singapore, een centrum van scheepsreparatie

Glasvezelversterkt polyester en de toepassing in

Het Keppel reparatiebedrijf en waarom het daar beter gaat

De Groninger Scheepsbouwproblematiek, in het kader van het College Werfinrichting en Werfbedrijf

Op zoek naar onderzoek...... voorbij de ivoren toren....: Een kleine verkenning rond het planologisch onderzoek