- document
-
Lameiras, F. (author), Heijnen, J.J. (author), Van Gulik, W.M. (author)In view of the high citric acid production capacity of Aspergillus niger, it should be well suited as a cell factory for the production of other relevant acids as succinic, fumaric, itaconic and malic. Quantitative metabolomics is an important omics tool in a synthetic biology approach to develop A. niger for the production of these acids. Such...journal article 2015
- document
-
De Jonge, L.P. (author), Douma, R.D. (author), Heijnen, J.J. (author), Van Gulik, W.M. (author)A sampling procedure for quantitative metabolomics in Penicillium chrysogenum based on cold aqueous methanol quenching was re-evaluated and optimized to reduce metabolite leakage during sample treatment. The optimization study included amino acids and intermediates of the glycolysis and the TCA-cycle. Metabolite leakage was found to be minimal...journal article 2011