Non-Bias-Limited Tracking of Spherical Particles, Enabling Nanometer Resolution at Low Magnification

We present a three-dimensional tracking routine for nondiffraction-limited particles, which significantly reduces pixel bias. Our technique allows for increased resolution compared to that of previous methods, especially at low magnification or at high signal/noise ratio. This enables tracking with nanometer accuracy in a wide field of view and...

Method for determining one or more characterizing features of a macromolecule and an apparatus for carrying out said method

The invention concerns a method and apparatus for determining one or more characterizing features of a macromolecule, in particular torque and/or twist of nucleic acids like DNA, using magnetic fields.

A method to track rotational motion for use in single-molecule biophysics

The double helical nature of DNA links many cellular processes such as DNA replication, transcription, and repair to rotational motion and the accumulation of torsional strain. Magnetic tweezers (MTs) are a single-molecule technique that enables the application of precisely calibrated stretching forces to nucleic acid tethers and to control...

Note: Interference technique for minimally invasive, subnanometer, microsecond measurements of displacements

We present a novel high-resolution technique for single-molecule experiments, viz., differential traveling wave tracking. This is an interference-based scattering technique where we use gold nanoparticles for high scattering intensities and incorporate differential measurements along one in-plane direction to subtract mechanical noise and drift...