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Wang, Zhongwei (author), Dunne, Aislinn (author), van Loosdrecht, Mark C.M. (author), Saikaly, Pascal E. (author)Saline wastewater is known to affect the performance of phosphate-accumulating organisms (PAOs) in enhanced biological phosphorus removal (EBPR) process. However, studies comparing the effect of salinity on different PAO clades are lacking. In this study, 'Candidatus Accumulibacter phosphatis' Clade I and II (hereafter referred to as PAOI and...journal article 2018
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Sorokin, Dimitry Y. (author), Merkel, Alexander Y. (author), Abbas, B.A. (author), Makarova, Kira S. (author), Rijpstra, W. Irene C. (author), Koenen, M. (author), Sinninghe Damsté, Jaap S. (author), Galinski, Erwin A. (author), Koonin, Eugene V. (author), van Loosdrecht, Mark C.M. (author)Methanogenic enrichments from hypersaline lakes at moderate thermophilic conditions have resulted in the cultivation of an unknown deep lineage of euryarchaeota related to the class Halobacteria. Eleven soda lake isolates and three salt lake enrichment cultures were methyl-reducing methanogens that utilize C<sub>1</sub> methylated compounds...journal article 2018
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Le, Quan H. (author), Verheijen, Peter J.T. (author), van Loosdrecht, Mark C.M. (author), Volcke, Eveline I.P. (author)A stepwise experimental design procedure to obtain reliable data from wastewater treatment plants (WWTPs) was developed. The proposed procedure aims at determining sets of additional measurements (besides available ones) that guarantee the identifiability of key process variables, which means that their value can be calculated from other,...journal article 2018
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Soler Jofra, A. (author), Wang, R. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author), Pérez, Julio (author)A lab-scale partial nitritation granular sludge air-lift reactor was operated in continuous mode treating low strength synthetic medium (influent ca. 50 mg-N-NH<sub>4</sub> <sup>+</sup>/L). Granules were initially stratified with AOB in the external shell and NOB in the inner core at 20 °C. Once temperature was decreased progressively from 20...journal article 2019
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Conthe Calvo, M. (author), Lycus, Pawel (author), Arntzen, Magnus (author), Ramos da Silva, Aline (author), Frostegård, Åsa (author), Bakken, Lars R. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)The strong greenhouse gas nitrous oxide (N<sub>2</sub>O) can be emitted from wastewater treatment systems as a byproduct of ammonium oxidation and as the last intermediate in the stepwise reduction of nitrate to N<sub>2</sub> by denitrifying organisms. A potential strategy to reduce N<sub>2</sub>O emissions would be to enhance the activity of...journal article 2019
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Sanawar, Huma (author), Bucs, Szilárd S. (author), Pot, Martin A. (author), Zlopasa, J. (author), Farhat, Nadia M. (author), Witkamp, Geert Jan (author), Kruithof, Joop C. (author), van Loosdrecht, Mark C.M. (author), Vrouwenvelder, J.S. (author)Routine chemical cleaning with the combined use of sodium hydroxide (NaOH) and hydrochloric acid (HCl) is carried out as a means of biofouling control in reverse osmosis (RO) membranes. The novelty of the research presented herein is in the application of urea, instead of NaOH, as a chemical cleaning agent to full-scale spiral-wound RO...journal article 2019
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Rombouts, J.L. (author), Mos, G. (author), Weissbrodt, D.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)A mechanistic understanding of microbial community establishment and product formation in open fermentative systems can aid the development of bioprocesses utilising organic waste. Kinetically, a single rate-limiting substrate is expected to result in one dominant species. Four enrichment cultures were operated to ferment either xylose or...journal article 2019
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Rubio Rincon, F.J. (author), Welles, L. (author), Lopez Vazquez, Carlos (author), Abbas, B.A. (author), van Loosdrecht, Mark C.M. (author), Brdjanovic, Damir (author)Candidatus Accumulibacter phosphatis is in general presented as the dominant organism responsible for the biological removal of phosphorus in activated sludge wastewater treatment plants. Lab-scale enhanced biological phosphorus removal (EBPR) studies, usually use acetate as carbon source. However, the complexity of the carbon sources present in...journal article 2019
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Suresh Kumar, P. (author), Korving, Leon (author), Keesman, Karel J. (author), van Loosdrecht, Mark C.M. (author), Witkamp, G.J. (author)Phosphate is a vital nutrient but its presence in surface waters even at very low concentrations can lead to eutrophication. Adsorption is often suggested as a step for reducing phosphate down to very low concentrations. Porous metal oxides can be used as granular adsorbents that have a high surface area and hence a high adsorption capacity....journal article 2019
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- Sorokin, Dimitry Y. (author), Makarova, Kira S. (author), Abbas, B.A. (author), Ferrer, Manuel (author), Golyshin, Peter N. (author), Galinski, Erwin A. (author), Ciorda, Sergio (author), Mena, María Carmen (author), van Loosdrecht, Mark C.M. (author) journal article 2019
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Jafari, Morez (author), Derlon, Nicolas (author), Desmond, Peter (author), van Loosdrecht, Mark C.M. (author), Morgenroth, Eberhard (author), Picioreanu, C. (author)Poroelastic fluid-structure interaction models were coupled to experimental data to determine the effects of biofilm spatial distribution of mechanical and hydraulic properties on the biofilm hydraulic resistance and compressibility in membrane filtration processes. Biofilms were cultivated on ultrafiltration membranes for 20 and 30 days...journal article 2019
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Felz, S. (author), Vermeulen, Pascalle (author), van Loosdrecht, Mark C.M. (author), Lin, Yue Mei (author)Biofilm structure and functionality depend on extracellular polymeric substances (EPS), but analytical methods for EPS often lack specificity which limits progress of biofilm research. EPS were extracted from aerobic granular sludge and analyzed with frequently applied colorimetric methods. The colorimetric methods were evaluated based on...journal article 2019
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de Graaff, D.R. (author), Felz, S. (author), Neu, Thomas R. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS). Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by...journal article 2019
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Olavarria, Karel (author), Fina, Albert (author), Velasco Alvarez, M.I. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)Biotechnological industry strives to develop anaerobic bioprocesses fueled by abundant and cheap carbon sources, like sucrose. However, oxygen-limiting conditions often lead to by-product formation and reduced ATP yields. While by-product formation is typically decreased by gene deletion, the breakdown of oligosaccharides with inorganic...journal article 2019
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Rubio Rincon, F.J. (author), Weissbrodt, D.G. (author), Lopez-Vazquez, C. M. (author), Welles, L. (author), Abbas, B.A. (author), Albertsen, M. (author), Nielsen, P. H. (author), van Loosdrecht, Mark C.M. (author), Brdjanovic, Damir (author)Populations of “Candidatus Accumulibacter”, a known polyphosphate-accumulating organism, within clade IC have been proposed to perform anoxic P-uptake activity in enhanced biological phosphorus removal (EBPR) systems using nitrate as electron acceptor. However, no consensus has been reached on the ability of “Ca. Accumulibacter” members of...journal article 2019
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Pallares Vega, R. (author), Blaak, Hetty (author), van der Plaats, Rozemarijn (author), de Roda Husman, Ana M. (author), Hernandez Leal, Lucia (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author), Schmitt, Heike (author)Wastewater treatment plants (WWTPs), linking human fecal residues and the environment, are considered as hotspots for the spread of antimicrobial resistance (AMR). In order to evaluate the role of WWTPs and underlying operational parameters for the removal of AMR, the presence and removal efficiency of a selected set of 6 antimicrobial...journal article 2019
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Boleij, M. (author), Seviour, Thomas (author), Wong, L.L.M. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Elucidating the extracellular polymeric substances (EPS) of anammox granular sludge is important for stable nitrogen removal processes in wastewater treatment. However, due to a lack of standardized methods for extraction and characterization, the composition of anammox granule EPS remains mostly unknown. In this study, alkaline (NaOH) and...journal article 2019
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Rombouts, J.L. (author), Mos, Galvin (author), Weissbrodt, D.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)Efficient industrial fermentation of lignocellulosic waste containing a large part of glucose and xylose is desirable to implement a circular economy. Mixed culture biotechnologies can aid in realizing this goal. The effect of feeding equivalent substrates to a microbial community, such a xylose and glucose, is not well understood in terms of...journal article 2019
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Prot, T.J.F. (author), Nguyen, V. H. (author), Wilfert, P.K. (author), Dugulan, A.I. (author), Goubitz, K. (author), de Ridder, D.J. (author), Korving, L. (author), Rem, P.C. (author), Bouderbala, A. (author), Witkamp, G.J. (author), van Loosdrecht, Mark C.M. (author)To prevent eutrophication of surface water, phosphate needs to be removed from sewage. Iron (Fe) dosing is commonly used to achieve this goal either as the main strategy or in support of biological removal. Vivianite (Fe(II) <sub>3</sub> (PO <sub>4</sub> ) <sub>2</sub> * 8H <sub>2</sub> O) plays a crucial role in capturing the phosphate, and...journal article 2019
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Kehrein, P.A. (author), van Loosdrecht, Mark C.M. (author), Osseweijer, P. (author), Posada Duque, J.A. (author)Municipal wastewater cannot any longer be perceived as a waste stream because it contains water, energy, fertilizer and other products that can be recovered with innovative technologies in so called ‘water resource factories’. Therefore a paradigm shift has been proclaimed to design water resource factories in the future that feed into a...journal article 2020