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Resch, V.A. (author), Jin, J. (author), Chen, B.S. (author), Hanefeld, U. (author)The Michael hydratase – alcohol dehydrogenase (MhyADH) from Alicycliphilus denitrificans was previously identified as a bi-functional enzyme performing a hydration of ?,?-unsaturated ketones and subsequent oxidation of the formed alcohols. The investigations of the bi-functionality were based on a spectrophotometric assay and an activity...journal article 2014
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Jin, J. (author), Straathof, A.J.J. (author), Pinkse, M.W.H. (author), Hanefeld, U. (author)A bifunctional hydratase/alcohol dehydrogenase was isolated from the cyclohexanol degrading bacterium Alicycliphilus denitrificans DSMZ 14773. The enzyme catalyzes the addition of water to ?,?-unsaturated carbonyl compounds and the subsequent alcohol oxidation. The purified enzyme showed three subunits in SDS gel, and the gene sequence revealed...journal article 2010