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Dekker, R. (author)The world is currently in a severe man-made climate crisis, creating the need for using renewable energy sources such as offshore wind farms. Offshore wind turbines do not take up any valuable space on land and can use the greater wind resource at sea. The downside is, however, that they are exposed to a much harsher environment at sea, which...doctoral thesis 2021
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Dekker, W.J.C. (author)Saccharomyces cerevisiae is a natural producer of ethanol and industrial strains can produce ethanol at high volumetric rates and near-theoretical yields. In addition to its fast fermentative metabolism, its GRAS (generally recognized as safe) status, ease of genetic engineering, tolerance to low pH and high ethanol concentrations contribute to...doctoral thesis 2021
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Dekker, Loek (author)In the Netherlands, one of the government bodies solving complex problems is the Netherlands Enterprise Agency (‘Rijksdienst Voor Ondernemers’; RVO). Within RVO, X-lab is developing new ways of working to be better prepared to deal with these complex problems. In short, X-lab is RVO’s internal innovation lab that creates and collects different...master thesis 2021
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Dekker, Hans (author)Oracles are mechanisms that provide blockchain networks with data that only exists outside of the network, such as asset prices. Decentralized Finance (DeFi) protocols use this data, and therefore their usability depends on the reliability of oracles. One such oracle system, widely used by DeFi protocols for pricing feeds, is Chainlink. The...bachelor thesis 2021
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Dekker, Jelle (author)In my graduation project I have researched the relation between built space and open space in the urban context. With the current densification challenge in Utrecht much more built volume is added to the city, increasing the pressure on open space. Open space is of great importance in securing urban liveability and has in particular many...master thesis 2021
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Kluba, M.M. (author), Li, J. (author), Parkkinen, Katja (author), Louwerse, Marcus (author), Snijder, Jaap (author), Dekker, R. (author)Several Silicon on Insulator (SOI) wafer manufacturers are now offering products with customer‐defined cavities etched in the handle wafer, which significantly simplifies the fabrication of MEMS devices such as pressure sensors. This paper presents a novel cavity buried oxide (BOX) SOI substrate (cavity‐BOX) that contains a patterned BOX...journal article 2021
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Dekker, Mireille (author), de Koning, Judith PM (author), Melles, M. (author), Sonneveld, M.H. (author), van Mansfeld, Rosa (author), Jongerden, Irene P (author)Background: Patient information provision about isolation measures is often not standardized. Moreover, it is unclear what information patients and informal caregivers need to optimize their knowledge and actual experience of contact isolation. We explored opportunities for improving the experience of patients in contact isolation from a human...journal article 2021
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- de Koning, Judith (author), Dekker, Mireille (author), Jongerden, Irene (author), Melles, M. (author) poster 2021
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Dekker, M.C. (author), van Egmond, R. (author), Molenbroek, J.F.M. (author), Vink, P. (author)BACKGROUND: Former studies on work-related upper limb disorders (WRULD) within university education report substantial prevalence rates. In this study, developments in WRULD amongst students in the period 2004-2014 were investigated. Our findings can be a benchmark for future studies, in particular when there are major societal changes as in...journal article 2021
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Bharadwaj, M. (author), Bengtson, M.L. (author), Golverdingen, Mirte (author), Waling, Loulotte (author), Dekker, C. (author)Inadequate and nonintegrated diagnostics are the Achilles' heel of global efforts to monitor, control, and eradicate neglected tropical diseases (NTDs). While treatment is often available, NTDs are endemic among marginalized populations, due to the unavailability or inadequacy of diagnostic tests that cause empirical misdiagnoses. The need of...review 2021
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Mooiman, C. (author), Bouwknegt, J. (author), Dekker, W.J.C. (author), Wiersma, S.J. (author), Ortiz Merino, R.A. (author), de Hulster, A.F. (author), Pronk, J.T. (author)All known facultatively fermentative yeasts require molecular oxygen for growth. Only in a small number of yeast species, these requirements can be circumvented by supplementation of known anaerobic growth factors such as nicotinate, sterols and unsaturated fatty acids. Biosynthetic oxygen requirements of yeasts are typically small and,...journal article 2021
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Perli, T. (author), Vos, A.M. (author), Bouwknegt, J. (author), Dekker, W.J.C. (author), Wiersma, S.J. (author), Mooiman, C. (author), Ortiz Merino, R.A. (author), Daran, J.G. (author), Pronk, J.T. (author)Neocallimastigomycetes are unique examples of strictly anaerobic eukaryotes. This study investigates how these anaerobic fungi bypass reactions involved in synthesis of pyridine nucleotide cofactors and coenzyme A that, in canonical fungal pathways, require molecular oxygen. Analysis of Neocallimastigomycetes proteomes identified a candidate...journal article 2021
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Hulleman, C.N. (author), Thorsen, R.Ø. (author), Kim, E. (author), Dekker, C. (author), Stallinga, S. (author), Rieger, B. (author)Estimating the orientation and 3D position of rotationally constrained emitters with localization microscopy typically requires polarization splitting or a large engineered Point Spread Function (PSF). Here we utilize a compact modified PSF for single molecule emitter imaging to estimate simultaneously the 3D position, dipole orientation, and...journal article 2021
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Fragasso, A. (author), de Vries, Hendrik W. (author), Andersson, John (author), van der Sluis, E.O. (author), van der Giessen, Erik (author), Dahlin, Andreas (author), Onck, Patrick R. (author), Dekker, C. (author)Nuclear Pore Complexes (NPCs) regulate bidirectional transport between the nucleus and the cytoplasm. Intrinsically disordered FG-Nups line the NPC lumen and form a selective barrier, where transport of most proteins is inhibited whereas specific transporter proteins freely pass. The mechanism underlying selective transport through the NPC is...journal article 2021
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Martins Da Ponte, R. (author), Gaio, N. (author), van Zeijl, H.W. (author), Vollebregt, S. (author), Dijkstra, Paul (author), Dekker, R. (author), Serdijn, W.A. (author), Giagka, Vasiliki (author)One of the many applications of organ-on-a-chip (OOC) technology is the study of biological processes in human induced pluripotent stem cells (iPSCs) during pharmacological drug screening. It is of paramount importance to construct OOCs equipped with highly compact in situ sensors that can accurately monitor, in real time, the extracellular...journal article 2021
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Yang, W.W.W. (author), van Dijk, M.C. (author), Primavera, Christian (author), Dekker, C. (author)We have developed a fabrication methodology for label-free optical trapping of individual nanobeads and proteins in inverted-bowtie-shaped plasmonic gold nanopores. Arrays of these nanoapertures can be reliably produced using focused ion beam (FIB) milling with gap sizes of 10–20 nm, single-nanometer variation, and with a remarkable stability...journal article 2021
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Janissen, R. (author), Woodman, Andrew (author), Shengjuler, Djoshkun (author), Vallet, Thomas (author), Lee, Kuo Ming (author), Kuijpers, L.C. (author), Moustafa, Ibrahim M. (author), Fitzgerald, Fiona (author), Dekker, N.H. (author)Viral RNA-dependent RNA polymerases (RdRps) are a target for broad-spectrum antiviral therapeutic agents. Recently, we demonstrated that incorporation of the T-1106 triphosphate, a pyrazine-carboxamide ribonucleotide, into nascent RNA increases pausing and backtracking by the poliovirus RdRp. Here, by monitoring enterovirus A-71 RdRp dynamics...journal article 2021
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Bohländer, P.R. (author), Filius, M. (author), van Kooten, Xander F. (author), Pomorski, A.K. (author), Schmid, S. (author), Dekker, C. (author), Eelkema, R. (author), Kim, S.H. (author), Joo, C. (author)Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside...review 2021
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Gogou, C. (author), Japaridze, A. (author), Dekker, C. (author)The process of DNA segregation, the redistribution of newly replicated genomic material to daughter cells, is a crucial step in the life cycle of all living systems. Here, we review DNA segregation in bacteria which evolved a variety of mechanisms for partitioning newly replicated DNA. Bacterial species such as Caulobacter crescentus and...review 2021
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Van de Cauter, L. (author), Fanalista, F. (author), van Buren, L. (author), De Franceschi, N. (author), Godino, E. (author), Bouw, Sharon (author), Danelon, C.J.A. (author), Dekker, C. (author), Koenderink, G.H. (author), Ganzinger, Kristina A. (author)Giant unilamellar vesicles (GUVs) are often used to mimic biological membranes in reconstitution experiments. They are also widely used in research on synthetic cells, as they provide a mechanically responsive reaction compartment that allows for controlled exchange of reactants with the environment. However, while many methods exist to...journal article 2021