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Dekker, W.J.C. (author), Wiersma, S.J. (author), Bouwknegt, J. (author), Mooiman, C. (author), Pronk, J.T. (author)In Saccharomyces cerevisiae, acyl-coenzyme A desaturation by Ole1 requires molecular oxygen. Tween 80, a poly-ethoxylated sorbitan-oleate ester, is therefore routinely included in anaerobic growth media as a source of unsaturated fatty acids (UFAs). During optimization of protocols for anaerobic bioreactor cultivation of this yeast, we...journal article 2019
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Liu, Y. (author), El Masoudi, Anissa (author), Pronk, J.T. (author), van Gulik, W.M. (author)So far, the physiology of Saccharomyces cerevisiae at near-zero growth rates has been studied in retentostat cultures with a growth-limiting supply of the carbon and energy source. Despite its relevance in nature and industry, the near-zero growth physiology of S. cerevisiae under conditions where growth is limited by the supply of non-energy...journal article 2019
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Else-Hassing, J. (author), de Groot, P.A. (author), Marquenie, Vita R. (author), Pronk, J.T. (author), Daran, J.G. (author)The organic compound 2-phenylethanol (2PE) has a pleasant floral scent and is intensively used in the cosmetic and food industries. Microbial production of 2PE by phenylalanine bioconversion or de novo biosynthesis from sugar offer sustainable, reliable and natural production processes compared to chemical synthesis. Despite the ability of...journal article 2019
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Gorter de Vries, A.R. (author), Pronk, J.T. (author), Daran, J.G. (author)The yeast Saccharomyces pastorianus is responsible for the annual worldwide production of almost 200 billion liters of lager-type beer. S. pastorianus is a hybrid of Saccharomyces cerevisiae and Saccharomyces eubayanus that has been studied for well over a century. Scientific interest in S. pastorianus intensified upon the discovery, in 2011,...journal article 2019
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Baldi, N. (author), Dykstra, James C. (author), Luttik, M.A.H. (author), Pabst, Martin (author), Wu, Liang (author), Benjamin, Kirsten R. (author), Vente, André (author), Pronk, J.T. (author), Mans, R. (author)Efficient production of fuels and chemicals by metabolically engineered micro-organisms requires availability of precursor molecules for product pathways. In eukaryotic cell factories, heterologous product pathways are usually expressed in the cytosol, which may limit availability of precursors that are generated in other cellular...journal article 2019
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Hakkaart, X.D.V. (author), Liu, Y. (author), Hulst, Mandy (author), el Masoudi, Anissa (author), Peuscher, Eveline (author), Pronk, J.T. (author), van Gulik, W.M. (author), Daran-Lapujade, P.A.S. (author)Engineered strains of Saccharomyces cerevisiae are used for industrial production of succinic acid. Optimal process conditions for dicarboxylic-acid yield and recovery include slow growth, low pH, and high CO<sub>2</sub>. To quantify and understand how these process parameters affect yeast physiology, this study investigates individual and...journal article 2019
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- Pronk, J.W. (author) doctoral thesis 2018
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Pronk, Robert (author)The study is the first to describe and model the dynamics and characteristics of communication during midstream modulation. A meta-model of seven communication traditions is deployed to frame the nature of communication. The study also validates the effectiveness of midstream modulation in the field of medical device technology.master thesis 2018
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Pronk, Anne (author)Measurements in the frequency range below 30 MHz, key to a better understanding of the origins of our universe, are one of the last unexplored areas of radio astronomy. Low frequency measurements suffer greatly from disturbance from man-made signals. Therefore, satellite missions are proposed which use the Moon as a shield from these...master thesis 2018
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Papapetridis, I. (author), Goudriaan, M. (author), Vazquez Vitali, M. (author), De Keijzer, Nikita A. (author), van den Broek, M.A. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)Background: Reduction or elimination of by-product formation is of immediate economic relevance in fermentation processes for industrial bioethanol production with the yeast Saccharomyces cerevisiae. Anaerobic cultures of wild-type S. cerevisiae require formation of glycerol to maintain the intracellular NADH/NAD<sup>+</sup> balance....journal article 2018
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Bracher, J.M. (author), Verhoeven, M.D. (author), Wisselink, H. Wouter (author), Crimi, B. (author), Nijland, Jeroen G. (author), Driessen, Arnold J.M. (author), Klaassen, Paul (author), van Maris, A.J.A. (author), Daran, J.G. (author), Pronk, J.T. (author)Background: l-Arabinose occurs at economically relevant levels in lignocellulosic hydrolysates. Its low-affinity uptake via the Saccharomyces cerevisiae Gal2 galactose transporter is inhibited by d-glucose. Especially at low concentrations of l-arabinose, uptake is an important rate-controlling step in the complete conversion of these...journal article 2018
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Mans, R. (author), Daran, J.G. (author), Pronk, J.T. (author)Evolutionary engineering, which uses laboratory evolution to select for industrially relevant traits, is a popular strategy in the development of high-performing yeast strains for industrial production of fuels and chemicals. By integrating whole-genome sequencing, bioinformatics, classical genetics and genome-editing techniques, evolutionary...review 2018
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Marques, W.L. (author), Mans, R. (author), Henderson, Ryan K. (author), Marella, Eko Roy (author), ter Horst, J. (author), de Hulster, A.F. (author), Poolman, Bert (author), Daran, J.G. (author), Pronk, J.T. (author), Gombert, Andreas K. (author), van Maris, A.J.A. (author)Anaerobic industrial fermentation processes do not require aeration and intensive mixing and the accompanying cost savings are beneficial for production of chemicals and fuels. However, the free-energy conservation of fermentative pathways is often insufficient for the production and export of the desired compounds and/or for cellular growth...journal article 2018
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Brickwedde, A. (author), Brouwers, N. (author), van den Broek, M.A. (author), Gallego Murillo, Joan Sebastián (author), Fraiture, J.L. (author), Pronk, J.T. (author), Daran, J.G. (author)Saccharomyces pastorianus lager brewing yeasts are domesticated hybrids of Saccharomyces cerevisiae and cold-tolerant Saccharomyces eubayanus. To understand the contribution of both parental genomes to maltose metabolism in brewing wort, this study focuses on maltose transport in the S. eubayanus type strain CBS 12357<sup>T</sup>/FM1318. To...journal article 2018
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Jürgens, H. (author), Varela, Javier A. (author), Gorter de Vries, A.R. (author), Perli, T. (author), Gast, V.J.M. (author), Gyurchev, N.Y. (author), Rajkumar, Arun S. (author), Mans, R. (author), Pronk, J.T. (author), Morrissey, John P. (author), Daran, J.G. (author)While CRISPR-Cas9-mediated genome editing has transformed yeast research, current plasmids and cassettes for Cas9 and guide-RNA expression are species specific. CRISPR tools that function in multiple yeast species could contribute to the intensifying research on non-conventional yeasts. A plasmid carrying a pangenomic origin of replication...journal article 2018
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Wijsman, M. (author), Swiat, M.A. (author), Marques, W.L. (author), Hettinga, Johanna K. (author), van den Broek, M.A. (author), Torre Cortés, Pilar de la (author), Mans, R. (author), Pronk, J.T. (author), Daran, J.G. (author), Daran-Lapujade, P.A.S. (author)Hexose transporter-deficient yeast strains are valuable testbeds for the study of sugar transport by native and heterologous transporters. In the popular Saccharomyces cerevisiae strain EBY.VW4000, deletion of 21 transporters completely abolished hexose transport. However, repeated use of the LoxP/Cre system in successive deletion rounds also...journal article 2018
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Jürgens, H. (author), Niemeijer, M.S. (author), Jennings-Antipov, Laura D. (author), Mans, R. (author), Morel, Jack (author), van Maris, A.J.A. (author), Pronk, J.T. (author), Gardner, Timothy S. (author)Open data in science requires precise definition of experimental procedures used in data generation, but traditional practices for sharing protocols and data cannot provide the required data contextualization. Here, we explore implementation, in an academic research setting, of a novel cloud-based software system designed to address this...journal article 2018
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de Graaff, D.R. (author), van Dijk, E.J.H. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)For a stable operation, the aerobic granular sludge process requires mechanically strong granules in balance with the shear forces in the reactor. Despite a wide general interest in granular stability, the mechanical strength of both anaerobic and aerobic granular sludge received very little attention. In this study, a high-shear method for...journal article 2018
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Bracher, J.M. (author), Martinez-Rodriguez, Oscar A. (author), Dekker, W.J.C. (author), Verhoeven, M.D. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)Expression of a heterologous xylose isomerase, deletion of the GRE3 aldose-reductase gene and overexpression of genes encoding xylulokinase (XKS1) and non-oxidative pentose-phosphate-pathway enzymes (RKI1, RPE1, TAL1, TKL1) enables aerobic growth of Saccharomyces cerevisiae on d-xylose. However, literature reports differ on whether anaerobic...journal article 2018
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Papapetridis, I. (author), Verhoeven, M.D. (author), Wiersma, S.J. (author), Goudriaan, M. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)Simultaneous fermentation of glucose and xylose can contribute to improved productivity and robustness of yeast-based processes for bioethanol production from lignocellulosic hydrolysates. This study explores a novel laboratory evolution strategy for identifying mutations that contribute to simultaneous utilisation of these sugars in batch...journal article 2018