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Tomas Martinez, S. (author), Kleikamp, H.B.C. (author), Neu, Thomas R. (author), Pabst, Martin (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Nonulosonic acids (NulOs) are a family of acidic carbohydrates with a nine-carbon backbone, which include different related structures, such as sialic acids. They have mainly been studied for their relevance in animal cells and pathogenic bacteria. Recently, sialic acids have been discovered as an important compound in the extracellular...journal article 2021
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Estevez Alonso, A. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Microbial community-based polyhydroxyalkanoate (PHA) production has been demonstrated repeatedly at pilot scale. Ammonium, normally present in waste streams, might be oxidized by nitrifying bacteria resulting in additional aeration energy demand. The use of low dissolved oxygen (DO) concentrations allowed to reduce nitrifying rates by up to...journal article 2021
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Kehrein, P.A. (author), van Loosdrecht, Mark C.M. (author), Osseweijer, P. (author), Posada Duque, J.A. (author)Municipal wastewater cannot any longer be perceived as a waste stream because it contains water, energy, fertilizer and other products that can be recovered with innovative technologies in so called ‘water resource factories’. Therefore a paradigm shift has been proclaimed to design water resource factories in the future that feed into a...journal article 2020
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Calderon Franco, D. (author), van Loosdrecht, Mark C.M. (author), Abeel, T.E.P.M.F. (author), Weissbrodt, D.G. (author)The free-floating extracellular DNA (exDNA) fraction of microbial ecosystems harbors antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs). Natural transformation of these xenogenetic elements can generate microbial cells resistant to one or more antibiotics. Isolating and obtaining high yield exDNA is challenging due to its low...journal article 2020
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van den Berg, L. (author), Kirkland, Catherine M. (author), Seymour, Joseph D. (author), Codd, Sarah L. (author), van Loosdrecht, Mark C.M. (author), de Kreuk, M.K. (author)Aerobic granular sludge (AGS) technology allows simultaneous nitrogen, phosphorus, and carbon removal in compact wastewater treatment processes. To operate, design, and model AGS reactors, it is essential to properly understand the diffusive transport within the granules. In this study, diffusive mass transfer within full-scale and lab-scale...journal article 2020
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Kim, Nam Kyeun (author), Mao, Ningtao (author), Lin, Richard (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)In this research, extracellular polymeric substances (EPS), such as EPS<sub>flocs</sub> and EPS<sub>granules</sub>, were successfully extracted from activated and aerobic granular sludge, respectively, and tested as bio-based flame retardant materials. Flax fabric was coated by the biopolymeric substances and its flammability was evaluated...journal article 2020
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Haaksman, V.A. (author), Mirghorayshi, M. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)Operational disturbances in aerobic granular sludge (AGS) systems can result in aerobic availability of readily biodegradable COD (rbCOD). Different from activated sludge, morphological consequences on the short and long term are not well described in literature. This study investigated the effect of incomplete anaerobic uptake of acetate on...journal article 2020
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Ochs, Pascal (author), Martin, Benjamin D. (author), Germain, Eve (author), Stephenson, Tom (author), van Loosdrecht, Mark C.M. (author), Soares, Ana (author)The benefits of deammonification to remove nitrogen from sidestreams, i.e., sludge dewatering liquors, in municipal wastewater treatment plants are well accepted. The ammonia removal from dewatering liquors originated from thermal hydrolysis/anaerobic digestion (THP/AD) are deemed challenging. Many different commercial technologies have been...journal article 2020
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Valk, L.C. (author), Diender, Martijn (author), Stouten, G.R. (author), Petersen, Jette F. (author), Nielsen, Per H. (author), Dueholm, Morten S. (author), Pronk, J.T. (author), van Loosdrecht, Mark C.M. (author)Acetogens have the ability to fixate carbon during fermentation by employing the Wood-Ljungdahl pathway (WLP), which is highly conserved across Bacteria and Archaea. In a previous study, product stoichometries in galacturonate-limited, anaerobic enrichment cultures of “Candidatus Galacturonibacter soehngenii,” from a novel genus within the...journal article 2020
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Valk, L.C. (author), Luttik, M.A.H. (author), de Ram, C. (author), Pabst, Martin (author), van den Broek, M.A. (author), van Loosdrecht, Mark C.M. (author), Pronk, J.T. (author)D-galacturonate, a key constituent of pectin, is a ubiquitous monomer in plant biomass. Anaerobic, fermentative conversion of D-galacturonate is therefore relevant in natural environments as well as in microbial processes for microbial conversion of pectin-containing agricultural residues. In currently known microorganisms that anaerobically...journal article 2020
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Rombouts, J.L. (author), Kranendonk, Elsemiek Madeleine Maria (author), Regueira, Alberte (author), Weissbrodt, D.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)Lactic acid-producing bacteria are important in many fermentations, such as the production of biobased plastics. Insight in the competitive advantage of lactic acid bacteria over other fermentative bacteria in a mixed culture enables ecology-based process design and can aid the development of sustainable and energy-efficient bioprocesses....journal article 2020
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Driessen, W. (author), Van Veldhoven, J. T.A. (author), Janssen, M. P.M. (author), van Loosdrecht, Mark C.M. (author)A long term operation (22 months) of the sidestream treatment plant at the water and resource recovery facility at the Tilburg sewage works in The Netherlands is presented. This plant treats dewatering reject liquor from thermally hydrolysed (THP) and mesophillic anaerobically digested (MAD) biosolids. The sidestream plant is comprised of a...journal article 2020
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Kulshreshtha, Y. (author), Mota, Nelson (author), Jagadish, Kaup S. (author), Bredenoord, Jan (author), Vardon, P.J. (author), van Loosdrecht, Mark C.M. (author), Jonkers, H.M. (author)There is an enormous demand for rural housing in India that needs to be catered for within a short span of time. Building with earth (mud) is proposed as an economical and environmental friendly alternative due to the rising costs of conventional building materials. However, the construction of earthen houses has significantly declined in...journal article 2020
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Calderon Franco, D. (author), Lin, Qingnan (author), van Loosdrecht, Mark C.M. (author), Abbas, B.A. (author), Weissbrodt, D.G. (author)The dissemination of DNA and xenogenic elements across waterways is under scientific and public spotlight due to new gene-editing tools, such as do-it-yourself (DIY) CRISPR-Cas kits deployable at kitchen table. Over decades, prevention of spread of genetically modified organisms (GMOs), antimicrobial resistances (AMR), and pathogens from...journal article 2020
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de Graaff, D.R. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)Many sources of wastewater contain sulfides, which can cause excessive growth of filamentous bacteria such as Thiothrix sp. resulting in bulking sludge in conventional activated sludge systems. Granular sludge systems could potentially also suffer from the growth of filamentous bacteria. Uptake of easily degradable COD by the relatively slow...journal article 2020
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Kehrein, P.A. (author), van Loosdrecht, Mark C.M. (author), Osseweijer, P. (author), Garfí, Marianna (author), Dewulf, Jo (author), Posada Duque, J.A. (author)In recent decades, academia has elaborated a wide range of technological solutions to recover water, energy, fertiliser and other products from municipal wastewater treatment plants. Drivers for this work range from low resource recovery potential and cost effectiveness, to the high energy demands and large environmental footprints of current...review 2020
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Kleikamp, H.B.C. (author), Lin, Y. (author), McMillan, D.G.G. (author), Geelhoed, Jeanine S. (author), Naus-Wiezer, Suzanne N.H. (author), Van Baarlen, Peter (author), Saha, Chinmoy (author), Louwen, Rogier (author), Sorokin, Dimitry Y. (author), van Loosdrecht, Mark C.M. (author), Pabst, Martin (author)Nonulosonic acids, commonly referred to as sialic acids, are a highly important group of nine-carbon sugars common to all domains of life. They all share biosynthetic and structural features, but otherwise display a remarkable chemical diversity. In humans, sialic acids cover all cells which makes them important for processes such as cellular...review 2020
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Barrios Hernandez, M.L. (author), Buenaño-Vargas, Claribel (author), Garcia, H. (author), Brdjanovic, Damir (author), van Loosdrecht, Mark C.M. (author), Hooijmans, Christine M. (author)The co-treatment of two synthetic faecal sludges (FS-1 and FS-2) with municipal synthetic wastewater (WW) was evaluated in an aerobic granular sludge (AGS) reactor. After characterisation, FS-1 showed the following concentrations, representative for medium-strength FS: 12,180 mg TSS L<sup>−1</sup>, 24,300 mg total COD L<sup>−1</sup>, 93.8 mg...journal article 2020
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Prot, T.J.F. (author), Wijdeveld, W. (author), Eshun, L. Ekua (author), Dugulan, A.I. (author), Goubitz, K. (author), Korving, L. (author), van Loosdrecht, Mark C.M. (author)The recovery of phosphorus from secondary sources like sewage sludge is essential in a world suffering from resources depletion. Recent studies have demonstrated that phosphorus can be magnetically recovered as vivianite (Fe(II)<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>∗8H<sub>2</sub>O) from the digested sludge (DS) of Waste Water Treatment...journal article 2020
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Boleij, M. (author), Kleikamp, H.B.C. (author), Pabst, Martin (author), Neu, Thomas R. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Anammox (anaerobic ammonium oxidation) bacteria are important for the nitrogen cycle in both natural environments and wastewater treatment plants. These bacteria have a strong tendency to grow in aggregates like biofilms and granular sludge. To understand the formation of anammox aggregates, it is required to unravel the composition of the...journal article 2020