Detection of Schistosoma haematobium using lensless imaging and flow cytometry, a proof of principle study

We present a simple method for the diagnosis of urinary schistosomiasis using an in-line lensless holographic microscope combined with flow cytometry technique. Using simple image processing algorithms and binary image classifier, our system provides automated detection of Schistosoma haematobium eggs in infected urine samples. Registered...

Effect of partial coherent illumination on Fourier Ptychography

Fourier Ptychography is a computational imaging technique able to decouple high resolution from wide field of view, bypassing the diffraction limit of the microscope. Since it does not rely on high precision mechanics or fluorescent imaging, it is of practical interest for implementation in low scale devices. Despite its gains, realizing a...

Imaging & identification of malaria parasites using cellphone microscope with a ball lens

We have optimized the design and imaging procedures, to clearly resolve the malaria parasite in Giemsa-stained thin blood smears, using simple low-cost cellphone-based microscopy with oil immersion. The microscope uses a glass ball as the objective and the phone camera as the tube lens. Our optimization includes the optimal choice of the ball...

Temperature-immune readout of an integrated optical wavelength meter based on microring resonators

Wavelength meters are central for many applications such as in telecommunication systems or laser monitoring. The primary function of a wavelength meter is to provide an output signal that changes sensitively with the wavelength of the input light. Of central importance is the reproducibility of the output signal even in the presence of external...

Aliasing, coherence, and resolution in a lensless holographic microscope

Temperature-drift-immune wavelength meter based on an integrated micro-ring resonator

Optical path difference microscopy with a Shack-Hartmann wavefront sensor

In this Letter, we show that a Shack-Hartmann wavefront sensor can be used for the quantitative measurement of the specimen optical path difference (OPD) in an ordinary incoherent optical microscope, if the spatial coherence of the illumination light in the plane of the specimen is larger than the microscope resolution. To satisfy this...

Sensorless adaptive optics system based on image second moment measurements

This paper presents experimental results of a static aberration control algorithm based on the linear relation be- tween mean square of the aberration gradient and the second moment of point spread function for the generation of control signal input for a deformable mirror (DM). Results presented in the work of Yang et al.1 suggested a good...

Wavefront coding with adaptive optics

We have implemented an extended depth of field optical system by wavefront coding with a micromachined membrane deformable mirror. This approach provides a versatile extension to standard wavefront coding based on fixed phase mask. First experimental results validate the feasibility of the use of adaptive optics for variable depth wavefront...