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Yarali, E. (author), Klimopoulou, M. (author), David, M.K. (author), Boukany, P. (author), Staufer, U. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author), Accardo, A. (author), Mirzaali, Mohammad J. (author)The Poisson's ratio and elastic modulus are two parameters determining the elastic behavior of biomaterials. While the effects of elastic modulus on the cell response is widely studied, very little is known regarding the effects of the Poisson's ratio. The micro-architecture of meta-biomaterials determines not only the Poisson's ratio but...journal article 2024
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Palepšienė, Rūta (author), Muralidharan, A. (author), Maciulevičius, Martynas (author), Ruzgys, Paulius (author), Chopra, S. (author), Boukany, P. (author), Šatkauskas, Saulius (author)RNA interference (RNAi) is a powerful and rapidly developing technology that enables precise silencing of genes of interest. However, the clinical development of RNAi is hampered by the limited cellular uptake and stability of the transferred molecules. Electroporation (EP) is an efficient and versatile technique for the transfer of both RNA...journal article 2024
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Muralidharan, A. (author), Boukany, P. (author)Electrotransfer of nucleic acids and proteins has become crucial in biotechnology for gene augmentation and genome editing. This review explores the applications of electrotransfer in both ex vivo and in vivo scenarios, emphasizing biomedical uses. We provide insights into completed clinical trials and successful instances of nucleic acid and...review 2024
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Rahman, Z. (author), Bordoloi, A.D.B. (author), Rouhana, Haifa (author), Tavasso, M. (author), van der Zon, Gerard (author), Garbin, V. (author), ten Dijke, Peter (author), Boukany, P. (author)Within the tumor microenvironment (TME), cancer cells use mechanotransduction pathways to convert biophysical forces to biochemical signals. However, the underlying mechanisms and functional significance of these pathways remain largely unclear. The upregulation of mechanosensitive pathways from biophysical forces such as interstitial flow (IF),...journal article 2023
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Boot, R.C. (author), Roscani, A.R. (author), van Buren, L. (author), Maity, Samadarshi (author), Koenderink, G.H. (author), Boukany, P. (author)Cell spheroids are in vitro multicellular model systems that mimic the crowded micro-environment of biological tissues. Their mechanical characterization can provide valuable insights in how single-cell mechanics and cell-cell interactions control tissue mechanics and self-organization. However, most measurement techniques are limited to...journal article 2023
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Lewis, R.W. (author), Muralidharan, A. (author), Klemm, B. (author), Boukany, P. (author), Eelkema, R. (author)Polycationic carriers promise low cost and scalable gene therapy treatments, however inefficient intracellular unpacking of the genetic cargo has limited transfection efficiency. Charge-reversing polycations, which transition from cationic to neutral or negative charge, can offer targeted intracellular DNA release. We describe a new class of...journal article 2023
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Mirzaie Yegane, M. (author), Boukany, P. (author), Zitha, P.L.J. (author)Due to increased energy demand, it is vital to enhance the recovery from existing oilfields. Polymer flooding is the most frequently used chemical enhanced oil recovery (cEOR) method in field applications that increases the oil sweep and displacement efficiencies. In recent years, there has been growing interest to assess the use of polymer...journal article 2022
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Mehta, P.P. (author), Rahman, Z. (author), ten Dijke, Peter (author), Boukany, P. (author)An early step of metastasis requires a complex and coordinated migration of invasive tumor cells into the surrounding tumor microenvironment (TME), which contains extracellular matrix (ECM). It is being appreciated that 3D matrix-based microfluidic models have an advantage over conventional in vitro and animal models to study tumor...review 2022
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Sinha, Abhishek (author), Mehta, P.P. (author), Fan, Chuannan (author), Zhang, Jing (author), Marvin, Dieuwke L. (author), van Dinther, Maarten (author), Ritsma, Laila (author), Boukany, P. (author), ten Dijke, Peter (author)Epithelial to mesenchymal transition (EMT) is crucial during embryonic development, tissue fibrosis, and cancer progression. Epithelial cells that display a cobblestone-like morphology can undergo a switch to mesenchymal-like phenotype, displaying an elongated spindle shape or a fibroblast-like morphology. EMT is characterized by timely and...book chapter 2022
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Zhang, Shuang (author), Tuk, Bastiaan (author), van de Peppel, Jeroen (author), Kremers, Gert Jan (author), Koedam, Marijke (author), Pesch, G.R. (author), Rahman, Z. (author), Hoogenboezem, Remco M. (author), Boukany, P. (author)A functional vascular system is a prerequisite for bone repair as disturbed angiogenesis often causes non-union. Paracrine factors released from human bone marrow derived mesenchymal stromal cells (BMSCs) have angiogenic effects on endothelial cells. However, whether these paracrine factors participate in blood flow dynamics within bone...journal article 2022
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Muralidharan, A. (author), Pesch, G.R. (author), Hubbe, H.M.K. (author), Rems, Lea (author), Nouri Goushki, M. (author), Boukany, P. (author)We developed a localized single-cell electroporation chip to deliver exogenous biomolecules with high efficiency while maintaining high cell viability. In our microfluidic device, the cells are trapped in a microtrap array by flow, after which target molecules are supplied to the device and electrotransferred to the cells under electric pulses....journal article 2022
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Scuderi, M. (author), Dermol-Černe, J. (author), Amaral da Silva, C. (author), Muralidharan, A. (author), Boukany, P. (author), Rems, Lea (author)Electroporation has become a powerful tool for nonviral delivery of various biomolecules such as nucleic acids, proteins, and chemotherapeutic drugs to virtually any living cell by exposing the cell membrane to an intense pulsed electric field. Different multiphysics and multiscale models have been developed to describe the phenomenon of...journal article 2022
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Liu, Q. (author), Muralidharan, A. (author), Saateh, A. (author), Ding, Z. (author), ten Dijke, Peter (author), Boukany, P. (author)In the research of cancer cell invasion and metastasis, recreation of physiologically relevant and faithful three-dimensional (3D) tumor models that recapitulate spatial architecture, spatiotemporal control of cell communication and signaling pathways, and integration of extracellular cues remains an open challenge. Here, a programmable...journal article 2022
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Boot, R.C. (author), Koenderink, G.H. (author), Boukany, P. (author)Spheroids are widely used in vitro 3D multicellular model systems that mimic complex physiological microenvironments of tissues. As different cell types vary in deformability and adhesion, the choice of (heterogeneous) cell composition will define overall spheroid mechanics, including their viscoelasticity and effective surface tension. These...review 2021
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Nouri Goushki, M. (author), Isaakidou, A. (author), Eijkel, B.I.M. (author), Minneboo, M.B. (author), Liu, Q. (author), Boukany, P. (author), Mirzaali, Mohammad J. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author)The surface topography of engineered extracellular matrices is one of the most important physical cues regulating the phenotypic polarization of macrophages. However, not much is known about the ways through which submicron (i.e., 100-1000 nm) topographies modulate the polarization of macrophages. In the context of bone tissue regeneration,...journal article 2021
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Mirzaie Yegane, M. (author), Schmidt, Julia (author), Dugonjic-Bilic, Fatima (author), Gerlach, Benjamin (author), Boukany, P. (author), Zitha, P.L.J. (author)We examine the role of preshearing on the flow properties of polymer solutions containing essentially an acrylamide-based copolymer obtained from an emulsified polymer emulsion inverted by a surfactant. The polymer solutions were presheared using three methods: (1) a Buddeberg disperser, (2) an Ultra-Turrax disperser, and (3) pressure-driven...journal article 2021
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Nouri Goushki, M. (author), Angeloni, L. (author), Modaresifar, K. (author), Minneboo, M.B. (author), Boukany, P. (author), Mirzaali, Mohammad J. (author), Ghatkesar, M.K. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author)The surface topography of implantable devices is of crucial importance for guiding the cascade of events that starts from the initial contact of the cells with the surface and continues until the complete integration of the device in its immediate environment. There is, however, limited quantitative information available regarding the...journal article 2021
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Muralidharan, A. (author), Rems, Lea (author), Kreutzer, M.T. (author), Boukany, P. (author)Transient physical disruption of cell membranes by electric pulses (or electroporation) has significance in biomedical and biological applications requiring the delivery of exogenous (bio)molecules to living cells. We demonstrate that actin networks regulate the cell membrane permeability during electroporation. Disruption of actin networks...journal article 2021
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Sachdev, S. (author), Feijoo Moreira, Sara (author), Keehnen, Yasmine (author), Rems, L. (author), Kreutzer, M.T. (author), Boukany, P. (author)Size of DNA molecules governs their interaction with the cell membrane during electroporation and their subsequent transport inside the cell. In order to investigate the effect of DNA size on DNA-membrane interaction during electroporation, cells are electro-pulsed with DNA molecules; 15 bp, 25 bp, 50 bp, 100 bp and 1000 bp (bp = base pairs)....journal article 2020
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Mirzaie Yegane, M. (author), Minaye Hashemi, F.S. (author), Vercauteren, Frank (author), Meulendijks, Nicole (author), Gharbi, Ridha (author), Boukany, P. (author), Zitha, P.L.J. (author)Water-soluble polyacrylamides have often been used to modify flow response in various water-based technologies and industrial processes, including paints, water treatment, paper manufacturing, and chemical enhanced oil recovery. Polymers are susceptible to degradation at combined high salinity and elevated temperature conditions which limits...journal article 2020
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