Engineering and characterization of alternative configurations of glycolysis in Saccharomyces cerevisiae

One of the most conserved and central parts of metabolism is the pathway of glycolysis, that breaks down hexose sugars. While multiple variants of glycolytic pathway exist, the Emden-Meyerhof-Parnas pathway which converts glucose to two molecules of pyruvate with the net gain of two ATP and two reduced NADH moieties is the one predominantly...

A systematic evaluation of yeast sample preparation protocols for spectral identifications, proteome coverage and post-isolation modifications

The importance of obtaining comprehensive and accurate information from cellular proteomics experiments asks for a systematic investigation of sample preparation protocols. In particular when working with unicellular organisms with strong cell walls, such as found in the model organism and cell factory Saccharomyces cerevisiae. Here, we...

Synthetic Genomics From a Yeast Perspective

Synthetic Genomics focuses on the construction of rationally designed chromosomes and genomes and offers novel approaches to study biology and to construct synthetic cell factories. Currently, progress in Synthetic Genomics is hindered by the inability to synthesize DNA molecules longer than a few hundred base pairs, while the size of the...

Full humanization of the glycolytic pathway in Saccharomyces cerevisiae

Although transplantation of single genes in yeast plays a key role in elucidating gene functionality in metazoans, technical challenges hamper humanization of full pathways and processes. Empowered by advances in synthetic biology, this study demonstrates the feasibility and implementation of full humanization of glycolysis in yeast. Single...

Improving Industrially Relevant Phenotypic Traits by Engineering Chromosome Copy Number in Saccharomyces pastorianus

The lager-brewing yeast Saccharomyces pastorianus is a hybrid between S. cerevisiae and S. eubayanus with an exceptional degree of aneuploidy. While chromosome copy number variation (CCNV) is present in many industrial Saccharomyces strains and has been linked to various industrially-relevant traits, its impact on the brewing performance of S...

Simulatie onderzoek naar de mogelijkheid van polarisatie metingen met een FM-CW radar.

In this report a presentation is given of the theory of polarisation measurements with radar. This theory is coupled to the characteristics of the Delft Atmospheric Research Radar and it is shown that full polarisation information can be acquired with this FM-CW radar in 6.25 ms. A simulation of the necessary signal processing is presented with...