Emergent Biological Endurance Depends on Extracellular Matrix Composition of Three-Dimensionally Printed Escherichia coli Biofilms

Biofilms are three-dimensional (3D) bacterial communities that exhibit a highly self-organized nature in terms of their composition and complex architecture. Bacteria in biofilms display emergent biological properties, such as resistance to antimicrobials and disinfectants that the individual planktonic cells lack. Bacterial biofilms possess...

Scalable bacterial production of moldable and recyclable biomineralized cellulose with tunable mechanical properties

Sustainable structural materials with excellent impact-resistance properties are urgently needed but challenging to produce, especially in a scalable fashion and with control over 3D shape. Here, we show that bacterial cellulose (BC) and bacterially precipitated calcium carbonate self-assemble into a layered structure reminiscent of tough...

Bioprinting of Regenerative Photosynthetic Living Materials

Living materials, which are fabricated by encapsulating living biological cells within a non-living matrix, have gained increasing attention in recent years. Their fabrication in spatially defined patterns that are mechanically robust is essential for their optimal functional performance but is difficult to achieve. Here, a bioprinting...

Biocompatible Graphene Oxide Nanosheets Densely Functionalized with Biologically Active Molecules for Biosensing Applications

Graphene oxide (GO) has immense potential for widespread use in diverse in vitro and in vivo biomedical applications owing to its thermal and chemical resistance, excellent electrical properties and solubility, and high surface-to-volume ratio. However, development of GO-based biological nanocomposites and biosensors has been hampered by its...

Bioproduced Polymers Self-Assemble with Graphene Oxide into Nanocomposite Films with Enhanced Mechanical Performance

Graphene oxide (GO) has recently been highlighted as a promising multipurpose two-dimensional material. However, free-standing graphene oxide films suffer from poor strength and flexibility, which limits scaling-up of production and lifetime structural robustness in applications. Inspired by the relationship between the organic and inorganic...

Bacterially Produced, Nacre-Inspired Composite Materials

The impressive mechanical properties of natural composites, such as nacre, arise from their multiscale hierarchical structures, which span from nano- to macroscale and lead to effective energy dissipation. While some synthetic bioinspired materials have achieved the toughness of natural nacre, current production methods are complex and...

Synthetic Biology for Multiscale Designed Biomimetic Assemblies: From Designed Self-Assembling Biopolymers to Bacterial Bioprinting

Nature is based on complex self-assembling systems that span from the nanoscale to the macroscale. We have already begun to design biomimetic systems with properties that have not evolved in nature, based on designed molecular interactions and regulation of biological systems. Synthetic biology is based on the principle of modularity,...

Creation of Conductive Graphene Materials by Bacterial Reduction Using Shewanella Oneidensis

Graphene's maximized surface-to-volume ratio, high conductance, mechanical strength, and flexibility make it a promising nanomaterial. However, large-scale graphene production is typically cost-intensive. This manuscript describes a microbial reduction approach for producing graphene that utilizes the bacterium Shewanella oneidensis in...

3D Printing for the Fabrication of Biofilm-Based Functional Living Materials

Bacterial biofilms are three-dimensional networks of cells entangled in a self-generated extracellular polymeric matrix composed of proteins, lipids, polysaccharides, and nucleic acids. Biofilms can establish themselves on virtually any accessible surface and lead to varying impacts ranging from infectious diseases to degradation of toxic...