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Hu, Chunyi (author), van Beljouw, S.P.B. (author), Nam, Ki Hyun (author), Schuler, Gabriel (author), Rodríguez Molina, A. (author), van Eijkeren-Haagsma, A.C. (author), Valk, M. (author), Pabst, Martin (author), Brouns, S.J.J. (author)The CRISPR-Cas type III-E RNA-targeting effector complex gRAMP/Cas7-11 is associated with a caspase-like protein (TPR-CHAT/Csx29) to form Craspase (CRISPR-guided caspase). Here, we use cryo-electron microscopy snapshots of Craspase to explain its target RNA cleavage and protease activation mechanisms. Target-guide pairing extending into the 5...journal article 2022
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van Beljouw, S.P.B. (author), van Eijkeren-Haagsma, A.C. (author), Rodriguez Molina, A. (author), van den Berg, D.F. (author), Vink, J.N.A. (author), Brouns, S.J.J. (author)Type III CRISPR-Cas immunity is widespread in prokaryotes and is generally mediated by multisubunit effector complexes. These complexes recognize complementary viral transcripts and can activate ancillary immune proteins. Here, we describe a type III-E effector from Candidatus “Scalindua brodae” (Sb-gRAMP), which is natively encoded by a...journal article 2021