Ester Formation

The present invention is in the field of a process for producing an ester, such as a biobased ester, from an aqueous biomass comprising solution, batch wise or continuously, wherein use of raw material is limited and if possible re-used. The present invention is in the field of green technology.

Transformation of biomass into commodity chemicals using enzymes or cells

Fermentative production of isobutene

Isobutene (2-methylpropene) is one of those chemicals for which bio-based production might replace the petrochemical production in the future. Currently, more than 10 million metric tons of isobutene are produced on a yearly basis. Even though bio-based production might also be achieved through chemocatalytic or thermochemical methods, this...

Purification, characterization, and cloning of a bifunctional molybdoenzyme with hydratase and alcohol dehydrogenase activity

A bifunctional hydratase/alcohol dehydrogenase was isolated from the cyclohexanol degrading bacterium Alicycliphilus denitrificans DSMZ 14773. The enzyme catalyzes the addition of water to ?,?-unsaturated carbonyl compounds and the subsequent alcohol oxidation. The purified enzyme showed three subunits in SDS gel, and the gene sequence revealed...

Theoretical performance of countercurrent reactors for production of enantiopure compounds

Irreversible reactions are being applied in enzymatic kinetic resolution to obtain enantiomerically pure compounds from racemic mixtures. Using model calculations for situations without mass transfer limitation, we show that reversible reactions might also be useful for enzymatic kinetic resolution, provided that countercurrent systems are used...

Hydrogel coated monoliths for enzymatic hydrolysis of penicillin G

The objective of this work was to develop a hydrogel-coated monolith for the entrapment of penicillin G acylase (E. coli, PGA). After screening of different hydrogels, chitosan was chosen as the carrier material for the preparation of monolithic biocatalysts. This protocol leads to active immobilized biocatalysts for the enzymatic hydrolysis of...

Fumaric acid production by fermentation

Abstract The potential of fumaric acid as a raw material in the polymer industry and the increment of cost of petroleum-based fumaric acid raises interest in fermentation processes for production of this compound from renewable resources. Although the chemical process yields 112% w/w fumaric acid from maleic anhydride and the fermentation...

Technical and Economical Feasibility of Production of Ethanol from Sugar Cane and Sugar Cane Bagasse

The primary aim of this study is to investigate and analyze the sugar-ethanol plants operating in Brazil to construct a raw model to gain better understanding and insight about the technical and economical aspects of the currently operating plants. And, the secondary aim is to combine the knowledge gathered from the currently existing plant with...

Primary amide synthesis from carboxylic acids with a lipase

The invention relates to a method of preparing a reaction product such as a primary amide, by means of two reagents A and B, which reagents form a salt that impedes fast conversion. The concentrations of the reagent A and the reagent B are chosen such that they comply with formula (I) wherein [A]T represents the concentration of the first...

Preparation of D-xylulose from D-xylose: Enzyme and Microbial Technology

Alkyl glycoside surfactants from starch and sucrose: Enzymic and chemical approaches