- document
-
Kleikamp, H.B.C. (author), Lin, Y. (author), McMillan, D.G.G. (author), Geelhoed, Jeanine S. (author), Naus-Wiezer, Suzanne N.H. (author), Van Baarlen, Peter (author), Saha, Chinmoy (author), Louwen, Rogier (author), Sorokin, Dimitry Y. (author), van Loosdrecht, Mark C.M. (author), Pabst, Martin (author)Nonulosonic acids, commonly referred to as sialic acids, are a highly important group of nine-carbon sugars common to all domains of life. They all share biosynthetic and structural features, but otherwise display a remarkable chemical diversity. In humans, sialic acids cover all cells which makes them important for processes such as cellular...review 2020
- document
-
Martens, Koen (author), van Beljouw, Sam P.B. (author), van der Els, Simon (author), Vink, J.N.A. (author), Baas, Sander (author), Vogelaar, George A. (author), Brouns, S.J.J. (author), van Baarlen, Peter (author), Kleerebezem, Michiel (author), Hohlbein, Johannes (author)CRISPR-Cas9 is widely used in genomic editing, but the kinetics of target search and its relation to the cellular concentration of Cas9 have remained elusive. Effective target search requires constant screening of the protospacer adjacent motif (PAM) and a 30 ms upper limit for screening was recently found. To further quantify the rapid...journal article 2019