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Kouba, Vojtěch (author), Hůrková, Kamila (author), Navrátilová, Klára (author), Kok, Dana (author), Benáková, Andrea (author), Laureni, M. (author), Vodičková, Patricie (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)The application of partial nitritation-anammox (PN/A) under mainstream conditions can enable substantial cost savings at wastewater treatment plants (WWTPs), but how process conditions and cell physiology affect anammox performance at psychrophilic temperatures below 15 °C remains poorly understood. We tested 14 anammox communities, including...journal article 2022
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Giulianetti de Almeida, M.P. (author), Mockaitis, Gustavo (author), Weissbrodt, D.G. (author)Milk discovery and processing enabled human settling and thriving in various settings. The discovery of cheese led to the production of whey as dairy by-product. Although it can find application in food, beverages, personal care products, pharmaceuticals and medical treatment, cheese whey is a massive dairying residue world-wide (154 Mm3·y-1)...journal article 2021
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Calderon Franco, D. (author), Apoorva, Seeram (author), Medema, G.J. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Wastewater treatment is challenged by the continuous emergence of chemical and biological contaminants. Disinfection, advanced oxidation, and activated carbon technologies are accessible in high-income countries to suppress them. Low-cost, easily implementable, and scalable solutions are needed for sanitation across regions. We studied the...journal article 2021
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Alloul, A. (author), Cerruti, M. (author), Adamczyk, Damian (author), Weissbrodt, D.G. (author), Vlaeminck, Siegfried E. (author)Purple non-sulfur bacteria (PNSB) show potential for microbial protein production on wastewater as animal feed. They offer good selectivity (i.e., low microbial diversity and high abundance of one species) when grown anaerobically in the light. However, the cost of closed anaerobic photobioreactors is prohibitive for protein production....journal article 2021
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Pallares Vega, R. (author), Macedo, Gonçalo (author), Brouwer, Michael S.M. (author), Hernandez Leal, Lucia (author), van der Maas, Peter (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author), Heederik, Dick (author), Mevius, Dik (author), Schmitt, Heike (author)Plasmid-mediated dissemination of antibiotic resistance among fecal Enterobacteriaceae in natural ecosystems may contribute to the persistence of antibiotic resistance genes in anthropogenically impacted environments. Plasmid transfer frequencies measured under laboratory conditions might lead to overestimation of plasmid transfer potential...journal article 2021
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Cerruti, M. (author), Guo, Bing (author), Delatolla, Robert (author), De Jonge, Nadieh (author), Hommes-De Vos Van Steenwijk, Aleida (author), Kadota, Paul (author), Mao, Ted (author), Oosterkamp, Margreet J. (author), Weissbrodt, D.G. (author)The wastewater treatment sector embraces mixed-culture biotechnologies for sanitation, environmental protection, and resource recovery. Bioprocess design, monitoring and control thrive on microbial processes selected in complex microbial communities. Microbial ecology and systems microbiology help access microbiomes and characterize...review 2021
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Pallares Vega, R. (author), Hernandez Leal, Lucia (author), Fletcher, Benjamin N. (author), Vias-Torres, Eduardo (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author), Schmitt, Heike (author)The occurrence and removal patterns of 24 antimicrobial agents and antimicrobial resistant determinants namely 6 antibiotic resistance genes (ARGs) and 2 mobile genetic elements (MGEs), and the fecal indicator E. coli were investigated in three full-scale wastewater treatment plants. Their waterlines and biosolids lines (including secondary...journal article 2021
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Tomas Martinez, S. (author), Kleikamp, H.B.C. (author), Neu, Thomas R. (author), Pabst, Martin (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Nonulosonic acids (NulOs) are a family of acidic carbohydrates with a nine-carbon backbone, which include different related structures, such as sialic acids. They have mainly been studied for their relevance in animal cells and pathogenic bacteria. Recently, sialic acids have been discovered as an important compound in the extracellular...journal article 2021
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Calderon Franco, D. (author), van Loosdrecht, Mark C.M. (author), Abeel, T.E.P.M.F. (author), Weissbrodt, D.G. (author)The free-floating extracellular DNA (exDNA) fraction of microbial ecosystems harbors antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs). Natural transformation of these xenogenetic elements can generate microbial cells resistant to one or more antibiotics. Isolating and obtaining high yield exDNA is challenging due to its low...journal article 2020
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Cerruti, M. (author), Stevens, B. (author), Ebrahimi, S. (author), Alloul, A. (author), Vlaeminck, Siegfried E. (author), Weissbrodt, D.G. (author)Mixed-culture biotechnologies are widely used to capture nutrients from wastewater. Purple non-sulfur bacteria (PNSB), a guild of anoxygenic photomixotrophic organisms, rise interest for their ability to directly assimilate nutrients in the biomass. One challenge targets the aggregation and accumulation of PNSB biomass to separate it from the...journal article 2020
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Rombouts, J.L. (author), Kranendonk, Elsemiek Madeleine Maria (author), Regueira, Alberte (author), Weissbrodt, D.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)Lactic acid-producing bacteria are important in many fermentations, such as the production of biobased plastics. Insight in the competitive advantage of lactic acid bacteria over other fermentative bacteria in a mixed culture enables ecology-based process design and can aid the development of sustainable and energy-efficient bioprocesses....journal article 2020
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Calderon Franco, D. (author), Lin, Qingnan (author), van Loosdrecht, Mark C.M. (author), Abbas, B.A. (author), Weissbrodt, D.G. (author)The dissemination of DNA and xenogenic elements across waterways is under scientific and public spotlight due to new gene-editing tools, such as do-it-yourself (DIY) CRISPR-Cas kits deployable at kitchen table. Over decades, prevention of spread of genetically modified organisms (GMOs), antimicrobial resistances (AMR), and pathogens from...journal article 2020
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Weissbrodt, D.G. (author), Winkler, M.K.H. (author), Wells, G.F. (author)Water resource recovery is central to the circular economy framework. It underlies the transition of environmental engineering from pollution prevention to responsible innovation for sustainable systems engineering. In order to speed this transition, resource recovery and circularity need integration into new higher education curricula to train...review 2020
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Laureni, M. (author), Weissbrodt, D.G. (author), Villez, Kris (author), Robin, Orlane (author), de Jonge, Nadieh (author), Rosenthal, Alex (author), Wells, George (author), Nielsen, Jeppe Lund (author), Morgenroth, Eberhard (author), Joss, Adriano (author)The control of nitrite-oxidizing bacteria (NOB) challenges the implementation of partial nitritation and anammox (PN/A) processes under mainstream conditions. The aim of the present study was to understand how operating conditions impact microbial competition and the control of NOB in hybrid PN/A systems, where biofilm and flocs coexist. A...journal article 2019
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Rombouts, J.L. (author), Mos, G. (author), Weissbrodt, D.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)A mechanistic understanding of microbial community establishment and product formation in open fermentative systems can aid the development of bioprocesses utilising organic waste. Kinetically, a single rate-limiting substrate is expected to result in one dominant species. Four enrichment cultures were operated to ferment either xylose or...journal article 2019
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Rubio Rincon, F.J. (author), Weissbrodt, D.G. (author), Lopez-Vazquez, C. M. (author), Welles, L. (author), Abbas, B.A. (author), Albertsen, M. (author), Nielsen, P. H. (author), van Loosdrecht, Mark C.M. (author), Brdjanovic, Damir (author)Populations of “Candidatus Accumulibacter”, a known polyphosphate-accumulating organism, within clade IC have been proposed to perform anoxic P-uptake activity in enhanced biological phosphorus removal (EBPR) systems using nitrate as electron acceptor. However, no consensus has been reached on the ability of “Ca. Accumulibacter” members of...journal article 2019
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Pallares Vega, R. (author), Blaak, Hetty (author), van der Plaats, Rozemarijn (author), de Roda Husman, Ana M. (author), Hernandez Leal, Lucia (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author), Schmitt, Heike (author)Wastewater treatment plants (WWTPs), linking human fecal residues and the environment, are considered as hotspots for the spread of antimicrobial resistance (AMR). In order to evaluate the role of WWTPs and underlying operational parameters for the removal of AMR, the presence and removal efficiency of a selected set of 6 antimicrobial...journal article 2019
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Rombouts, J.L. (author), Mos, Galvin (author), Weissbrodt, D.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)Efficient industrial fermentation of lignocellulosic waste containing a large part of glucose and xylose is desirable to implement a circular economy. Mixed culture biotechnologies can aid in realizing this goal. The effect of feeding equivalent substrates to a microbial community, such a xylose and glucose, is not well understood in terms of...journal article 2019
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Fumasoli, Alexandra (author), Bürgmann, Helmut (author), Weissbrodt, D.G. (author), Wells, G.N. (author), Beck, Karin (author), Mohn, Joachim (author), Morgenroth, Eberhard (author), Udert, Kai M. (author)Ammonia oxidation decreases the pH in wastewaters where alkalinity is limited relative to total ammonia. The activity of ammonia oxidizing bacteria (AOB), however, typically decreases with pH and often ceases completely in slightly acidic wastewaters. Nevertheless, nitrification at low pH has been reported in reactors treating human urine,...journal article 2017
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Prest, E.I.E.D. (author), Weissbrodt, D.G. (author), Hammes, F (author), van Loosdrecht, Mark C.M. (author), Vrouwenvelder, J.S. (author)Large seasonal variations in microbial drinking water quality can occur in distribution networks, but are often not taken into account when evaluating results from short-term water sampling campaigns. Temporal dynamics in bacterial community characteristics were investigated during a two-year drinking water monitoring campaign in a full-scale...journal article 2016