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Zelle, R.M. (author), De Hulster, E. (author), Van Winden, W.A. (author), De Waard, P. (author), Dijkema, C. (author), Winkler, A.A. (author), Geertman, J.M. (author), Van Dijken, J.P. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)Malic acid is a potential biomass-derivable "building block" for chemical synthesis. Since wild-type Saccharomyces cerevisiae strains produce only low levels of malate, metabolic engineering is required to achieve efficient malate production with this yeast. A promising pathway for malate production from glucose proceeds via carboxylation of...journal article 2008
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Heijnen, J.J. (author), Daran, J.M. (author), Pronk, J.T. (author), Daran-Lapujade, P. (author), Knijnenburg, T.A. (author), Ras, C. (author), Ten Pierick, A. (author), Akmering, M.J. (author), Van Winden, W.A. (author), Kresnowati, M.T. (author)Within the first 5 min after a sudden relief from glucose limitation, Saccharomyces cerevisiae exhibited fast changes of intracellular metabolite levels and a major transcriptional reprogramming. Integration of transcriptome and metabolome data revealed tight relationships between the changes at these two levels. Transcriptome as well as...journal article 2006