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Vermeer, C.M. (author), Bons, Larissa J. (author), Kleerebezem, R. (author)Abstract: Using microbial enrichment cultures for the production of waste-derived polyhydroxyalkanoates (PHAs) is a promising technology to recover secondary resources. Volatile fatty acids (VFAs) form the preferred substrate for PHA production. Isobutyrate is a VFA appearing in multiple waste valorization routes, such as anaerobic...journal article 2022
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Vermeer, C.M. (author), Depaz, Lena (author), van den Berg, Emily (author), Koelmans, Tom (author), Kleerebezem, R. (author)Medium-chain-length polyhydroxyalkanoate (mcl-PHA) production by using microbial enrichments is a promising but largely unexplored approach to obtain elastomeric biomaterials from secondary resources. In this study, several enrichment strategies were tested to select a community with a high mcl-PHA storage capacity when feeding octanoate. On...journal article 2022
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Tamis, J. (author), Joosse, B.M. (author), de Leeuw, K.D. (author), Kleerebezem, R. (author)In this study, we investigated the operational performance and product spectrum of glucose-fermenting anaerobic granular sludge reactor at pH 4. A selective environment for the growth of granules was implemented by the introduction of a 2 min settling phase, a hydraulic retention time of 6 h and a solid retention time of 12 ± 3 days. The...journal article 2021
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Rombouts, J.L. (author), Kranendonk, Elsemiek Madeleine Maria (author), Regueira, Alberte (author), Weissbrodt, D.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)Lactic acid-producing bacteria are important in many fermentations, such as the production of biobased plastics. Insight in the competitive advantage of lactic acid bacteria over other fermentative bacteria in a mixed culture enables ecology-based process design and can aid the development of sustainable and energy-efficient bioprocesses....journal article 2020
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Marang, L. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author)The process for non-axenic polyhydroxyalkanoate (PHA) production from organic waste generally comprises three steps: acidogenic fermentation of the waste stream, enrichment of a PHA-producing culture, and production of the PHA. This study assesses the feasibility of combining the enrichment and production step. Harvesting PHA-rich biomass...journal article 2016
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Mooij, P.R. (author), De Jongh, L.D. (author), Van Loosdrecht, M.C.M. (author), Kleerebezem, R. (author)Microalgae have the potential to supply a biobased society with essential feedstocks like sugar and lipids. Besides being productive, strains used for this purpose should grow fast, be resistant to predators, and have good harvestability properties. Diatoms, a class of siliceous algae, have these and other preferred characteristics. In this...journal article 2015
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Tamis, J. (author), Sorokin, D.Y. (author), Jiang, Y. (author), Van Loosdrecht, M.C.M. (author), Kleerebezem, R. (author)Background Many waste streams have a relatively high vegetable oil content, which is a potential resource that should be recovered. Microbial storage compound production for the recovery of lipids from lipid-water emulsions with open (unsterilized) microbial cultures was investigated in a sequencing batch reactor using a diluted vegetable oil...journal article 2015
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Jiang, Y. (author), Mikova, G. (author), Kleerebezem, R. (author), Van der Wielen, L.A.M. (author), Cuellar Soares, M.C. (author)This study focused on investigating the feasibility of purifying polyhydroxybutyrate (PHB) from mixed culture biomass by alkaline-based chemical treatment. The PHB-containing biomass was enriched on acetate under non-sterile conditions. Alkaline treatment (0.2 M NaOH) together with surfactant SDS (0.2 w/v% SDS) could reach 99% purity, with more...journal article 2015