Preventing overfitting in Mixed Integer Optimization based classification tree construction

The global use of azole antifungals as treatment against infections caused by <i>Candida</i> has led to an increase in azole resistance. The primal goal of this BSc thesis is to improve existing Mixed Integer Optimization models to classify azole resistance of <i>C. glabrata</i> more accurately by preventing overfitting. Moreover, these...

Cross-linked enzyme aggregates (CLEAs): Stable and recyclable biocatalysts

The key to obtaining an optimum performance of an enzyme is often a question of devising an effective method for its immobilization. In the present review, we describe a novel, versatile and effective methodology for enzyme immobilization as CLEAs (cross-linked enzyme aggregates). The method is exquisitely simple (involving precipitation of the...

Heat flux measurements for the fast monitoring of dynamic responses to glucose additions by yeasts that were subjected to different feeding regimes in continuous culture

Effects of oxygen limitation on sugar metabolism in yeasts: A continuous-culture study of the Kluyver effect

Localization and kinetics of pyruvate-metabolizing enzymes in relation to aerobic alcoholic fermentation in Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621

Phosphorus-31 nuclear magnetic resonance studies of intracellular pH, phosphate compartmentation and phosphate transport in yeasts

31p NMR spectra were obtained from suspensions of Candida utilis, Saccharomyces cerevisiae and Zygosaccharomyces bailii grown aerobically on glucose. Direct introduction of substrate into the cell suspension, without interruption of the measurements, revealed rapid changes in pH upon addition of the energy source. All 3 ~p NMR spectra of the...