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Development of a patient-specific FE model of the knee to study cartilage degeneration following ACL reconstruction: A sensitivity analysis to evaluate the effects of cartilage and meniscal Young's moduli on cartilage degeneration

Talsma, Maaike (author)

Osteoarthritis (OA) is a progressive joint degeneration disease resulting in joint pain, stiffness, and loss of mobility. Among patients with anterior cruciate ligament reconstruction (ACLR), OA incidence is increased. Articular cartilage (AC) degeneration is irreversible, therefore prevention and early detection are essential. Specifically, the...

master thesis 2023

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Engineered biochemical cues of regenerative biomaterials to enhance endogenous stem/progenitor cells (ESPCs)-mediated articular cartilage repair

Zhou, Liangbin (author), Xu, J. (author), Schwab, Andrea (author), Tong, Wenxue (author), Zheng, Lizhen (author), Li, Zhuo (author), Xu, Shunxiang (author), van Osch, G.J.V.M. (author), Wen, Chunyi (author), Qin, Ling (author)

As a highly specialized shock-absorbing connective tissue, articular cartilage (AC) has very limited self-repair capacity after traumatic injuries, posing a heavy socioeconomic burden. Common clinical therapies for small- to medium-size focal AC defects are well-developed endogenous repair and cell-based strategies, including microfracture,...

review 2023

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Mesenchymal stem/stromal cells-derived extracellular vesicles as a potentially more beneficial therapeutic strategy than MSC-based treatment in a mild metabolic osteoarthritis model

Warmink, Kelly (author), Rios, Jaqueline L. (author), Varderidou-Minasian, Suzy (author), Torres-Torrillas, Marta (author), van Valkengoed, Devin R. (author), Versteeg, Sabine (author), Eijkelkamp, Niels (author), Weinans, Harrie (author), Korthagen, Nicoline M. (author), Lorenowicz, Magdalena J. (author)

Background: Mesenchymal stromal/stem cells (MSCs) and MSC-derived extracellular vesicles (MSC-EVs) hold promise as a disease modifying treatment in osteoarthritis (OA). Obesity, and its associated inflammation, contribute to OA development and metabolic OA represents a specific and significant group of the OA patient population. Given their...

journal article 2023

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Rapid Whole-Knee Quantification of Cartilage Using T ₁, T ₂ ^∗, and T _RAFF2 Mapping With Magnetic Resonance Fingerprinting

Tourais, Joao (author), Ploem, T.W. (author), Zadelhoff, Tijmen A.van (author), Steeg-Henzen, Christal van de (author), Oei, Edwin H.G. (author), Weingärtner, S.D. (author)

Objective: Quantitative Magnetic Resonance Imaging (MRI) holds great promise for the early detection of cartilage deterioration. Here, a Magnetic Resonance Fingerprinting (MRF) framework is proposed for comprehensive and rapid quantification of T ₁, T ₂ ^∗, and T _RAFF2 with whole-knee coverage....

journal article 2023

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Senescence during early differentiation reduced the chondrogenic differentiation capacity of mesenchymal progenitor cells

Voskamp, Chantal (author), Koevoet, Wendy J.L.M. (author), van Osch, G.J.V.M. (author), Narcisi, Roberto (author)

Introduction: Mesenchymal stromal/progenitor cells (MSCs) are promising for cartilage cell-based therapies due to their chondrogenic differentiation capacity. However, MSCs can become senescent during in vitro expansion, a state characterized by stable cell cycle arrest, metabolic alterations, and substantial changes in the gene expression and...

journal article 2023

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Modulating design parameters to drive cell invasion into hydrogels for osteochondral tissue formation

Schwab, Andrea (author), Wesdorp, Marinus A. (author), Xu, J. (author), Abinzano, Florencia (author), Loebel, Claudia (author), Levato, Riccardo (author), Eglin, David (author), Narcisi, Roberto (author), Malda, Jos (author), van Osch, G.J.V.M. (author)

Background: The use of acellular hydrogels to repair osteochondral defects requires cells to first invade the biomaterial and then to deposit extracellular matrix for tissue regeneration. Due to the diverse physicochemical properties of engineered hydrogels, the specific properties that allow or even improve the behaviour of cells are not yet...

journal article 2023

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3D printed and punched porous surfaces of a non-resorbable, biphasic implant for the repair of osteochondral lesions improves repair tissue adherence and ingrowth

Fugazzola, Maria C. (author), Golafshan, Nasim (author), van Aken, Joris A. (author), Plomp, S. (author), De Grauw, Janny (author), van Buul, Ward (author), Hermsen, Gied (author), Weinans, Harrie (author), Castilho, Miguel (author), van Weeren, René (author), Malda, Jos (author)

Summary: The objective of this study was to evaluate a non-resorbable implant for the focal repair of osteochondral defects. Enhanced adherence of repair cartilage overgrowing the implants was the secondary goal and was tested by introducing porosities on the articular surface of the implant. This study evaluated four versions of the...

journal article 2023

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Functionalized Hydrogels for Articular Cartilage Tissue Engineering

Zhou, Liangbin (author), Guo, Peng (author), D'Este, Matteo (author), Tong, Wenxue (author), Xu, Jiankun (author), Yao, Hao (author), Stoddart, Martin J. (author), van Osch, G.J.V.M. (author), Ho, Kevin Ki Wai (author), Li, Zhen (author), Qin, Ling (author)

Articular cartilage (AC) is an avascular and flexible connective tissue located on the bone surface in the diarthrodial joints. AC defects are common in the knees of young and physically active individuals. Because of the lack of suitable tissue-engineered artificial matrices, current therapies for AC defects, especially full-thickness AC...

review 2022

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Modulation of Inflamed Synovium Improves Migration of Mesenchymal Stromal Cells in Vitro Through Anti-Inflammatory Macrophages

Wesdorp, Marinus A. (author), Bastiaansen-Jenniskens, Yvonne M. (author), Capar, Serdar (author), Verhaar, Jan A.N. (author), Narcisi, R. (author), van Osch, G.J.V.M. (author)

OBJECTIVE: Inflammation is known to negatively affect cartilage repair. However, it is unclear how inflammation influences the migration of mesenchymal stromal cells (MSCs) from the underlying bone marrow into the defect. We therefore aimed to investigate how synovial inflammation influences MSC migration, and whether modulation of...

journal article 2022

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Osteoarthritis year in review 2021: mechanics

Harlaar, J. (author), Macri, E.M. (author), Wesseling, M.G.H. (author)

Osteoarthritis (OA) has a complex, heterogeneous and only partly understood etiology. There is a definite role of joint cartilage pathomechanics in originating and progressing of the disease. Although it is still not identified precisely enough to design or select targeted treatments, the progress of this year's research demonstrates that...

review 2022

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Intra-articular Administration of Triamcinolone Acetonide in a Murine Cartilage Defect Model Reduces Inflammation but Inhibits Endogenous Cartilage Repair

Wesdorp, Marinus A. (author), Capar, Serdar (author), Bastiaansen-Jenniskens, Yvonne M. (author), Kops, Nicole (author), Creemers, Laura B. (author), Verhaar, Jan A.N. (author), van Osch, G.J.V.M. (author), Wei, Wu (author)

Background: Cartilage defects result in joint inflammation. The presence of proinflammatory factors has been described to negatively affect cartilage formation. Purpose: To evaluate the effect and timing of administration of triamcinolone acetonide (TAA), an anti-inflammatory drug, on cartilage repair using a mouse model. Study Design:...

journal article 2022

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4D bioprinting for cartilage tissue engineering: a controlled shape transformation approach

Kalogeropoulou, Maria (author)

Cartilage degeneration is a major cause of chronic disability and its treatment usually involves highly invasive procedures. To address this issue, three-dimensional (3D) bioprinting was introduced as a promising tissue engineering approach. However, the structures fabricated with this method are static and lack the dynamic response of native...

master thesis 2021

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The Development of a Cartilage Specific Bioink for Three-Dimensional Bioprinting

Kingma, Esther (author)

Bioprinting is a promising technique that has the ability to generate complex tissue structures for articular cartilage (AC) repair and regeneration. However, most biomaterials used for bioprinting are incapable of representing the complexity of native extracellular matrix (ECM). In this study, a novel cartilage specific bioink has been...

master thesis 2021

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4D Bioprinting: Temporal Stiffening of Mesenchymal Stem Cell-Laden Hyaluronic Acid-Alginate Scaffolds for Cartilage Constructs

Berkhout, Willemijn (author)

Three-dimensional (3D) bioprinting studies the initial condition of printed constructs, which makes construct static, and neglects the dynamic changes in natural tissue conformation during tissue repair, disease progression and regeneration processes. Therefore, the concept of four-dimensional (4D) bioprinting has been introduced that allows for...

master thesis 2021

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Bioprinting of a Zonal-Specific Cell Density Scaffold: A Biomimetic Approach for Cartilage Tissue Engineering

Dimaraki, A. (author), Diaz Payno, P.J. (author), Minneboo, M.B. (author), Nouri Goushki, M. (author), Hosseini, M. (author), Kops, N. (author), Narcisi, R. (author), Mirzaali, Mohammad J. (author), van Osch, G.J.V.M. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author)

The treatment of articular cartilage defects remains a significant clinical challenge. This is partially due to current tissue engineering strategies failing to recapitulate native organization. Articular cartilage is a graded tissue with three layers exhibiting different cell densities: the superficial zone having the highest density and the...

journal article 2021

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The Releasate of Avascular Cartilage Demonstrates Inherent Pro-Angiogenic Properties In Vitro and In Vivo

Nossin, Yannick (author), Farrell, Eric (author), Koevoet, Wendy J.L.M. (author), Datema, Frank (author), Somoza, Rodrigo A. (author), Caplan, Arnold I. (author), van Osch, G.J.V.M. (author)

Objective: Cartilage is avascular and numerous studies have identified the presence of single anti- and pro-angiogenic factors in cartilage. To better understand the maintenance hyaline cartilage, we assessed the angiogenic potential of complete cartilage releasate with functional assays in vitro and in vivo. Design: We evaluated the gene...

journal article 2021

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Dual-contrast computed tomography enables detection of equine posttraumatic osteoarthritis in vitro

Saukko, Annina E.A. (author), Nykänen, Olli (author), Sarin, Jaakko K. (author), Nissi, Mikko J. (author), te Moller, Nikae C.R. (author), Weinans, Harrie (author), Mancini, Irina A.D. (author), Visser, Jetze (author), Brommer, Harold (author)

To prevent the progression of posttraumatic osteoarthritis, assessment of cartilage composition is critical for effective treatment planning. Posttraumatic changes include proteoglycan (PG) loss and elevated water content. Quantitative dual-energy computed tomography (QDECT) provides a means to diagnose these changes. Here, we determine the...

journal article 2021

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Bioprinting of Zonal Cartilage Scaffolds Using Different Cell Densities: A biomimetic approach on cartilage regeneration

Dimaraki, Angeliki (author)

The use of allografts for the treatment of critical size cartilage defects holds disadvantages including the limited number of donors and the compromised chondrocyte viability. These limitations have prompted researchers to explore different options, such as cartilage engineering. Bioprinting, a promising tissue engineering technique, could be...

master thesis 2020

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Dual contrast in computed tomography allows earlier characterization of articular cartilage over single contrast

Bhattarai, Abhisek (author), Pouran, Behdad (author), Mäkelä, Janne T.A. (author), Shaikh, Rubina (author), Honkanen, Miitu K.M. (author), Prakash, Mithilesh (author), Kröger, Heikki (author), Grinstaff, Mark W. (author), Weinans, Harrie (author), Jurvelin, Jukka S. (author), Töyräs, Juha (author)

Cationic computed tomography contrast agents are more sensitive for detecting cartilage degeneration than anionic or non-ionic agents. However, osteoarthritis-related loss of proteoglycans and increase in water content contrarily affect the diffusion of cationic contrast agents, limiting their sensitivity. The quantitative dual-energy...

journal article 2020

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Mechanical stress inhibits early stages of endogenous cell migration: A pilot study in an ex vivo osteochondral model

Vainieri, Maria L. (author), Alini, Mauro (author), Yayon, Avner (author), van Osch, G.J.V.M. (author), Grad, Sibylle (author)

Cell migration has a central role in osteochondral defect repair initiation and biomaterial-mediated regeneration. New advancements to reestablish tissue function include biomaterials and factors promoting cell recruitment, differentiation and tissue integration, but little is known about responses to mechanical stimuli. In the present pilot...

journal article 2020