Looping the Genome with SMC Complexes

SMC (structural maintenance of chromosomes) protein complexes are an evolutionarily conserved family of motor proteins that hold sister chromatids together and fold genomes throughout the cell cycle by DNA loop extrusion. These complexes play a key role in a variety of functions in the packaging and regulation of chromosomes, and they have...

Transport receptor occupancy in nuclear pore complex mimics

Nuclear pore complexes (NPCs) regulate all molecular transport between the nucleus and the cytoplasm in eukaryotic cells. Intrinsically disordered Phe-Gly nucleoporins (FG-Nups) line the central conduit of NPCs to impart a selective barrier where large proteins are excluded unless bound to a transport receptor (karyopherin; Kap). Here, we...

Adapting the process of a public sector innovation lab with the help of design thinking: The case of X-lab Rvo

In the Netherlands, one of the government bodies solving complex problems is the Netherlands Enterprise Agency (‘Rijksdienst Voor Ondernemers’; RVO). Within RVO, X-lab is developing new ways of working to be better prepared to deal with these complex problems. In short, X-lab is RVO’s internal innovation lab that creates and collects different...

A next step in disruption management: combining operations research and complexity science

Railway systems occasionally get into a state of being out-of-control, meaning that barely any train is running, even though the required resources (infrastructure, rolling stock and crew) are available. Because of the large number of affected resources and the absence of detailed, timely and accurate information, currently existing...

Distinct Roles for Condensin's Two ATPase Sites in Chromosome Condensation

Condensin is a conserved SMC complex that uses its ATPase machinery to structure genomes, but how it does so is largely unknown. We show that condensin's ATPase has a dual role in chromosome condensation. Mutation of one ATPase site impairs condensation, while mutating the second site results in hyperactive condensin that compacts DNA faster...

A Next Step in Disruption Management: Combining Operations Research and Complexity Science

Railway systems occasionally get into a state of out-of-control, meaning that there is barely any train is running, even though the required resources (infrastructure, rolling stock and crew) are available. These situations can either be caused by large disruptions or unexpected propagation and accumulation of delays. Because of the large number...

Activated dynamics in the two-dimensional Ising spin-glass Rb2Cu1-xCo xF4