Multi-omic analyses in immune cell development with lessons learned from T cell development

Traditionally, flow cytometry has been the preferred method to characterize immune cells at the single-cell level. Flow cytometry is used in immunology mostly to measure the expression of identifying markers on the cell surface, but—with good antibodies—can also be used to assess the expression of intracellular proteins. The advent of single...

Supervised machine learning in microfluidic impedance flow cytometry for improved particle size determination

The assessment of particle and cell size in electrical microfluidic flow cytometers has become common practice. Nevertheless, in flow cytometers with coplanar electrodes accurate determination of particle size is difficult, owing to the inhomogeneous electric field. Pre-defined signal templates and compensation methods have been introduced to...

Comparing the bacterial growth potential of ultra-low nutrient drinking water assessed by growth tests based on flow cytometric intact cell count versus adenosine triphosphate

The bacterial growth potential (BGP) of drinking water is widely assessed either by flow cytometric intact cell count (BGP_ICC) or adenosine triphosphate (BGP_ATP) based methods. Combining BGP_ICC and BGP_ATP measurements has been previously applied for various types of drinking water having high to low...

Bacterial community dynamics and disinfection impact in cooling water systems

Understanding the bacterial dynamics in cooling towers is imperative for the assessment of disinfection efficiency and management of microbial risks linked to aerosol formation. The objective of this study was to evaluate the impact of feed water on the cooling water bacterial microbiome and investigate the survival ability of its members...

Digital holography integrated with flow cytometry for detection of urinary schistosomiasis

Schistosomiasis is an intravascular infection with major public health consequences in developing countries. It is one of the major Neglected Tropical Diseases with more than 240 million people infected and 800 million people at risk in 2015, mostly in sub-Saharan Africa. It is caused by trematode parasites of the genus Schistosoma, in this...

Further developing the bacterial growth potential method for ultra-pure drinking water produced by remineralization of reverse osmosis permeate

Ensuring the biological stability of drinking water is essential for modern drinking water supply. To understand and manage the biological stability, it is critical that the bacterial growth in drinking water can be measured. Nowadays, advance treatment technologies, such as reverse osmosis (RO), are increasingly applied in drinking water...

Visual analysis of mass cytometry data by hierarchical stochastic neighbour embedding reveals rare cell types

Mass cytometry allows high-resolution dissection of the cellular composition of the immune system. However, the high-dimensionality, large size, and non-linear structure of the data poses considerable challenges for the data analysis. In particular, dimensionality reduction-based techniques like t-SNE offer single-cell resolution but are...

Biological stability of drinking water: Controlling factors, methods, and challenges

Biological stability of drinking water refers to the concept of providing consumers with drinking water of same microbial quality at the tap as produced at the water treatment facility. However, uncontrolled growth of bacteria can occur during distribution in water mains and premise plumbing, and can lead to hygienic (e.g., development of...

Biological stability in drinking water distribution systems: A novel approach for systematic microbial water quality monitoring

Challenges to achieve biological stability in drinking water distribution systems Drinking water is distributed from the treatment facility to consumers through extended man-made piping systems. The World Health Organization drinking water guidelines (2006) stated that “Water entering the distribution system must be microbiologically safe and...

Miniaturized flow cytometer with 3D hydrodynamic particle focusing and integrated optical elements applying silicon photodiodes

In this study, the design, realization and measurement results of a novel optofluidic system capable of performing absorbance-based flow cytometric analysis is presented. This miniaturized laboratory platform, fabricated using SU-8 on a silicon substrate, comprises integrated polymer-based waveguides for light guiding and a biconcave cylindrical...