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Bastiaanssen, C.K.J.M.L. (author)Interactions are essential to life. At the cellular level, the most fundamental processes rely on interactions between DNA, RNA, and proteins. Given that the structure and function of these molecules are determined by their sequence, it is ultimately the sequence that dictates the strength and specificity of these interactions. Thus, to improve...doctoral thesis 2024
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Orbons, Sander (author)Future Very High-Throughput Satellites are foreseen to implement optical ground-to-satellite feeder links to achieve multi-terabit-per-second data rates. Optical links however, are highly susceptible to atmospheric losses caused by turbulence, absorption, and scattering, especially at low elevation angles. Despite prior confirmation of the...master thesis 2024
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Picanço Castanheira Da Silva, T. (author), Eppink, M.H.M. (author), Ottens, M. (author)The monoclonal antibody (mAb) industry is becoming increasingly digitalized. Digital twins are becoming increasingly important to test or validate processes before manufacturing. High-Throughput Process Development (HTPD) has been progressively used as a tool for process development and innovation. The combination of High-Throughput Screening...journal article 2024
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Zuidema, W. (author)In this thesis, the design and engineering considerations for a multi-beam scanning electron microscope (MBSEM) are discussed. This microscope can benefit biological research in various ways. It can give new insights into the inner workings of a multitude of biological systems that were hard to get using previously existing instrumentation. For...doctoral thesis 2023
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Hashemi, A. (author)In this thesis, I highlighted a number of projects aimed at developing and testing new simulation methods for studying complex reactive systems, with a particular emphasis on simulation strategies based on the concept of bonding graphs. These mathematical structures provide useful tools for a variety of algorithms developed over the last few...doctoral thesis 2023
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Esselman, Allison B. (author), Patterson, Nathan Heath (author), Migas, L.G. (author), Dufresne, Martin (author), Djambazova, Katerina V. (author), Colley, Madeline E. (author), Van de Plas, Raf (author), Spraggins, Jeffrey M. (author)The glomerulus is a multicellular functional tissue unit (FTU) of the nephron that is responsible for blood filtration. Each glomerulus contains multiple substructures and cell types that are crucial for their function. To understand normal aging and disease in kidneys, methods for high spatial resolution molecular imaging within these FTUs...journal article 2023
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Mangnus, Mark J.J. (author), de Wit, Jur W. (author), Vonk, Sander J.W. (author), Geuchies, J.J. (author), Albrecht, Wiebke (author), Bals, Sara (author), Houtepen, A.J. (author), Rabouw, Freddy T. (author)In recent years, quantum dots (QDs) have emerged as bright, color-tunable light sources for various applications such as light-emitting devices, lasing, and bioimaging. One important next step to advance their applicability is to reduce particle-to-particle variations of the emission properties as well as fluctuations of a single QD’s...journal article 2023
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Abbassi-Daloii, Tooba (author), el Abdellaoui, Salma (author), Kan, H.E. (author), van den Akker, E.B. (author), ’t Hoen, Peter A.C. (author), Raz, Vered (author), Voortman, Lenard M. (author)Skeletal muscles are composed of different myofiber types characterized by the expression of myosin heavy chain isoforms, which can be affected by physical activity, aging, and pathological conditions. Here, we present a step-by-step high-throughput semi-automated approach for performing myofiber type quantification of entire human or mouse...journal article 2023
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Postma, J.I. (author), Ferrari, A. (author), Bottger, A.J. (author)Surface compositions play a predominant role in the efficiency and lifetime of membranes and catalysts. The surface composition can change during operation due to segregation, thus controlling and predicting the surface composition is essential. Computational modelling can aid in predicting alloy stability, along with designing surface alloys...journal article 2023
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Picanço Castanheira Da Silva, T. (author), Eppink, Michel (author), Ottens, M. (author)Biopharmaceuticals are becoming increasingly important in modern healthcare. Monoclonal antibodies (mAb) are one of the most widely used therapeutic proteins and are important for the treatment of cancer and autoimmune diseases, among others. After cell culture there are still large amounts of other impurities (e.g. host cell proteins) in...journal article 2022
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Wang, Feifei (author), Pan, Jiazheng (author), Hu, Yulin (author), Zhou, Jie (author), Wang, Haoqian (author), Huang, Xin (author), Chu, Wenhai (author), van der Hoek, J.P. (author)Biological activated carbon (BAC) filtration is usually considered to be able to decrease formation potentials (FPs) of disinfection by-products (DBPs) in drinking water treatment plant (DWTP). However, BAC filters with long running time may release microbial metabolites to effluents and therefore increase FPs of nitrogenous DBPs with high...journal article 2022
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Severins, I.W.H. (author), Joo, C. (author), van Noort, John (author)Next-generation sequencing techniques have led to a new quantitative dimension in the biological sciences. In particular, integrating sequencing techniques with biophysical tools allows sequence-dependent mechanistic studies. Using the millions of DNA clusters that are generated during sequencing to perform high-throughput binding affinity...review 2022
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Barrios, Alan J. (author), Peron, Deizi V. (author), Chakkingal, Anoop (author), Dugulan, A.I. (author), Moldovan, Simona (author), Nakouri, Kalthoum (author), Thuriot-Roukos, Joëlle (author), Wojcieszak, Robert (author), Thybaut, Joris W. (author)Hydrogenation into light olefins is an attractive strategy for CO<sub>2</sub>fixation into chemicals. In this article, high throughput experimentation and extended characterization were employed to identify the most efficient promoters and to elucidate structure-performance correlations and reaction paths in the CO<sub>2</sub>hydrogenation to...journal article 2022
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Keulen, D. (author), Geldhof, Geoffroy (author), Bussy, Olivier Le (author), Pabst, Martin (author), Ottens, M. (author)The safety requirements for vaccines are extremely high since they are administered to healthy people. For that reason, vaccine development is time-consuming and very expensive. Reducing time-to-market is key for pharmaceutical companies, saving lives and money. Therefore the need is raised for systematic, general and efficient process...journal article 2022
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FLOROS, Theofanis (author)Current high-end medical X-ray intervention devices provide a tremendous amount of high-definition images per second. Combined with the additional inputs from the numerous auxiliary devices, processing and compositing the data in real-time quickly becomes an arduous engineering challenge. Furthermore, the critical nature of this application...master thesis 2021
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Antoniadis, Gregory (author)Ammonia is an important chemical used in the production of fertilizers and has the potential to become a green energy carrier. It is synthesized through the Haber-Bosch (HB) process, which involves nitrogen and hydrogen reacting at high temperature and pressure and consumes approximately 2% of the global energy supply. An alternative to this...master thesis 2021
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Bera, Subhas Chandra (author), Seifert, Mona (author), Kirchdoerfer, Robert N. (author), van Nies, Pauline (author), Wubulikasimu, Yibulayin (author), Quack, Salina (author), Papini, Flávia S. (author), Depken, S.M. (author), Dulin, D. (author)Coronaviruses have evolved elaborate multisubunit machines to replicate and transcribe their genomes. Central to these machines are the RNA-dependent RNA polymerase subunit (nsp12) and its intimately associated cofactors (nsp7 and nsp8). We use a high-throughput magnetic-tweezers approach to develop a mechanochemical description of this core...journal article 2021
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Seifert, Mona (author), Bera, Subhas C. (author), van Nies, Pauline (author), Kirchdoerfer, Robert N. (author), Shannon, Ashleigh (author), Le, Thi Tuyet Nhung (author), Meng, Xiangzhi (author), Depken, S.M. (author), Dulin, David (author)The absence of 'shovel-ready' anti-coronavirus drugs during vaccine development has exceedingly worsened the SARS-CoV-2 pandemic. Furthermore, new vaccine-resistant variants and coronavirus outbreaks may occur in the near future, and we must be ready to face this possibility. However, efficient antiviral drugs are still lacking to this day,...journal article 2021
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Moreno Gonzalez, M. (author), Chuekitkumchorn, Pattra (author), Silva, Marcelo (author), Groenewoud, Roos (author), Ottens, M. (author)Proteins derived from plant resources such as oilseed meals, canola and sunflower, are considered a viable alternative to animal proteins for food consumption. This work presents a rational methodology, using high throughput experimentation (HTE), for the separation of cruciferin and napin, the two major proteins of canola meal, by...journal article 2021
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Picanço Castanheira Da Silva, T. (author), Eppink, Michel (author), Ottens, M. (author)Process development in the biotech industry leads to investments around hundred of millions of dollars. It is important to mitigate costs without neglecting the quality of process development. Biopharmaceutical process development is important for companies to develop new processes and be first to market, improve a pre-established process, or...review 2021