A robotic platform to screen aqueous two-phase systems for overcoming inhibition in enzymatic reactions

Aqueous two-phase systems (ATPS) can be applied to enzymatic reactions that are affected by product inhibition. In the biorefinery context, sugars inhibit the cellulolytic enzymes in charge of converting the biomass. Here, we present a strategy to select an ATPS (formed by polymer and salt) that can separate sugar and enzymes. This automated...

In-situ product removal by membrane extraction

In bioproduction processes of chemicals and pharmaceuticals, downstream processing usually is a significant cost factor. The products require a high purity (especially biopharmaceutical products), therefore, the process usually contains a large number of separation steps. Moreover, the high costs in downstream processing are caused by the fact...