3D Microelectrode Integration in Muscle-on-Chip device

The current drug development process is a costly effort both in terms of budget and time. Organ-on-Chip (OoC) technologies are rapidly developing and are used for various applications and studies that aim to reduce the costs of this process. They represent the convergence between tissue engineering and microfluidic technology to provide...

Two-photon polymerization-based 3D-multi-electrode arrays for electrical monitoring of neuronal cells

3D-multi-electrode arrays (3D-MEAs) are needed to overcome the limitations of 2D-multi-electrode arrays (2D-MEAs) and enable the electrical characterization of 3D neuronal cultures in in vitro brain models, advancing the understanding of neurological disorders and paving the way to personalized medicine. The aim of this thesis was to overcome...

OoC-integrated impedance spectroscopy for blood-brain barrier integrity analysis

The lack of reliable human physiology models in vitro combined with an ever-increasing set of health and safety requirements imposed by pharmaceutical regulatory agencies across the world is causing a concerningly low number of new drugs to reach the market. Organ-on-Chip (OoC) technology aims to aid faster development of new drugs by providing...

Portable and Integrated Organ-On-Chip Platform Using Off-the-shelf Components

Due to the developments of microfluidics, Organ-On-Chip (OOC) technology is developing rapidly in recent years. Microflow plays a vital role in OOC applications, thus a microfluidic platform that can generate high-quality flow is always needed. However, the existing OOC systems contain bulky peripheral components, incompact design and low...

A 3D microelectrode array to record neural activity at different tissue depths

Measuring neuronal responses along all three spatial dimensions is the next step, beyond the capacity of established 2D microelectrode arrays (MEAs), to record electric activity inside tissues with highly accurate spatial resolution. This research presents silicon-based planar arrays of 3D microelectrodes, whereby each microelectrode supports...

Optimization of microporous PDMS membranes for Organ-on-Chip devices

Organ-on-chip (OOC) devices are micro-engineered three-dimensional (3D) biomimetic systems that can be used to create in vitro models of human tissue. They provide an alternative for conventional cell culture tools in pharmaceutical R&D. Moreover, these devices can contribute in research focused on understanding complex disease processes....