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Deng, Shaoyu (author), Liu, J. (author), Yang, Xiaofan (author), Sun, Dezhi (author), Wang, Aijie (author), van Loosdrecht, Mark C.M. (author), Cheng, Xiang (author)The accumulation of phosphorus in activated sludge in wastewater treatment plants (WWTPs) provides potential for phosphorus recovery from sewage. This study delves into the potential for releasing phosphorus from waste activated sludge through two distinct treatment methods—thermal hydrolysis and pH adjustment. The investigation was conducted...journal article 2024
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Martins, Tiago A.E. (author), Muñoz Sierra, Julian (author), Nieuwlands, Jo A. (author), Lousada Ferreira, M. (author), Amaral, Leonor (author)The ecotoxicological safety of the water bodies relies on the reduction of micropollutant emissions from wastewater treatment plants (WWTP). The ecotoxicological safety of the water bodies relies on the reduction of micropollutant emissions from wastewater treatment plants (WWTP). Quantification of micropollutant removal at full-scale WWTP is...journal article 2024
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Kosar, Sadiye (author), Isik, Onur (author), Cicekalan, Busra (author), Gulhan, Hazal (author), Cingoz, Seyma (author), Yoruk, Mustafa (author), Ozgun, Hale (author), van Loosdrecht, Mark C.M. (author), Ersahin, Mustafa Evren (author)Achieving a neutral/positive energy balance without compromising discharge standards is one of the main goals of wastewater treatment plants (WWTPs) in terms of sustainability. Aerobic granular sludge (AGS) technology promises high treatment performance with low energy and footprint requirement. In this study, high-rate activated sludge (HRAS...journal article 2023
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Hu, Zhi Yi (author), Lin, Yi Peng (author), Wang, Qing Ting (author), Zhang, Yi Xin (author), Tang, Jie (author), Wang, Shuai (author), van Loosdrecht, Mark C.M. (author), Zeng, Raymond Jianxiong (author), Zhang, Fang (author)By maintaining the cell integrity of waste activated sludge (WAS), structural extracellular polymeric substances (St-EPS) resist WAS anaerobic fermentation. This study investigates the occurrence of polygalacturonate in WAS St-EPS by combining chemical and metagenomic analyses that identify ∼22% of the bacteria, including Ferruginibacter and...journal article 2023
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Han, Heejoo (author), Kim, Daehyun D. (author), Song, Min Joon (author), Yun, Taeho (author), Yoon, Hyun (author), Lee, Hong Woon (author), Kim, Young Mo (author), Laureni, M. (author), Yoon, S.Y. (author)Wastewater treatment plants (WWTPs) are a major source of N<sub>2</sub>O, a potent greenhouse gas with 300 times higher global warming potential than CO<sub>2</sub>. Several approaches have been proposed for mitigation of N<sub>2</sub>O emissions from WWTPs and have shown promising yet only site-specific results. Here, self-sustaining...journal article 2023
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Li, Ji (author), Hao, Xiaodi (author), Shen, Zhan (author), Wu, Yuanyuan (author), van Loosdrecht, Mark C.M. (author)A high moisture content of waste activated sludge (WAS) associated with a low calorific value needs to be deeply dried towards self-supporting incineration. On the other hand, thermal energy with low temperature exchanged from treated effluent has great potential for drying sludge. Unfortunately, low-temperature drying of sludge seems to be...journal article 2023
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Pei, R. (author), Vicente-Venegas, Gerard (author), Tomaszewska-Porada, Agnieszka (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Polyhydroxyalkanoates (PHAs) can be produced with municipal waste activated sludge from biological wastewater treatment processes. Methods of selective fluorescent staining with confocal laser scanning microscopy (CLSM) were developed and optimized to evaluate the distribution of PHA storage activity in this mixed culture activated sludge...journal article 2023
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Pei, R. (author), Tarek-Bahgat, N. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Poly(3-hydroxybutyrate) (PHB) was accumulated in full-scale municipal waste activated sludge at pilot scale. After accumulation, the fate of the PHB-rich biomass was evaluated over two weeks as a function of initial pH (5.5, 7.0 and 10), and incubation temperature (25, 37 and 55 °C), with or without aeration. PHB became consumed under aerobic...journal article 2023
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Wang, Xiangyang (author), Shi, Chen (author), Hao, Xiaodi (author), van Loosdrecht, Mark C.M. (author), Wu, Yuanyuan (author)Wet-chemical approach is widely applied for phosphate recovery from incinerated ash of waste activated sludge (WAS), along with metals removed/recovered. The high contents of both aluminum (Al) and iron (Fe) in WAS-incinerated ash should be suitable for producing coagulants with some waste anions like Cl<sup>−</sup> and SO<sub>4</sub><sup>2−<...journal article 2023
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Estevez Alonso, A. (author)Microbes are the gears that sustain all forms of life in planet Earth and are expected to become key players in the transition towards a more sustainable society. In this thesis, microbes from sewage sludge are used for the production of polyhydroxyalkanoates, biodegradable polymers that have the potential to replace<br/>many of the petroleum...doctoral thesis 2022
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WANG, Zhouyuan (author)Thermal hydrolysis (TH) has been widely applied to sewage sludge treatment to improve downstream anaerobic digestion (AD) by enhancing organic solids hydrolysis and subsequent biogas productivity. However, TH also has several drawbacks, including the formation of recalcitrant compounds, which are anaerobically non-biodegradable and even...master thesis 2022
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Mathematical Modelling of the Hydrolysis Process of Waste Activated Sludge in a Novel Cascade SystemDurry, Sebastian (author)The hydrolysis of sludge solids especially for difficultly degradable sludges such as WAS is not fully understood, yet. The first-order hydrolysis rate was shown to function well for most easily degradable sludges and soluble substrates. This description for substrate hydrolysis in the context of anaerobic digestion has the benefit of being very...master thesis 2022
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Xu, Yanghui (author), Ou, Qin (author), He, Qiang (author), Wu, Zhengsong (author), Ma, Jun (author), Huangfu, Xiaoliu (author)The extracellular polymeric substances (EPS) from activated sludge played significant roles in the removal of nanoparticles from wastewater. A series of batch experiments were carried out to determine the adsorption mechanism of three nano-Ag by activated sludge, as well as the contributions of EPS fractions including dissolved EPS (DEPS),...journal article 2022
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Chen, Xingyu (author), Lee, Yu Jen (author), Yuan, Tian (author), Lei, Zhongfang (author), Adachi, Yasuhisa (author), Zhang, Zhenya (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author)A reasonable recovery of excess sludge may shift the waste into wealth. Recently an increasing attention has been paid to the recycling of extracellular biopolymers from conventional and advanced biological wastewater treatment systems such as flocculent activated sludge (AS), bacterial aerobic granular sludge (AGS), and algal-bacterial AGS...review 2022
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Estevez Alonso, A. (author), Arias-Buendía, María (author), Pei, R. (author), van Veelen, H. Pieter J. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Activated sludge from municipal wastewater treatment processes can be used directly for the production of biodegradable polyesters from the family of polyhydroxyalkanoates (PHAs). However, municipal activated sludge typically cannot accumulate PHAs to very high levels and often low yields of polymer produced on substrate are observed. In the...journal article 2022
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Estevez Alonso, A. (author), Altamira-Algarra, Beatriz (author), Arnau-Segarra, César (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)The developments of mixed culture polyhydroxyalkanoate production has been directed to maximize the biomass PHA content with limited attention to polymer quality. Direct comparison of PHA accumulation literature is challenging, and even regularly contradicting in reported results, due to underlying differences that are not well expressed. A...journal article 2022
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Sharma, Keshab (author), Gutierrez, Oriol (author), Yuan, Zhiguo (author), Daelman, Matthijs R.J. (author), van Loosdrecht, Mark C.M. (author), Volcke, Eveline I.P. (author)This chapter provides a review of the models available for estimating the production and emission of methane from wastewater collection and treatment systems. The details of a number of mechanistic models as well as the simplified empirical models have been summarized. Their limitations have been identified and general methods for calibration...book chapter 2022
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Pei, R. (author), Vicente-Venegas, Gerard (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Polyhydroxyalkanoate accumulation experiments at pilot scale were performed with fullscale municipal waste activated sludge. Development of biomass PHA content was quantified by thermogravimetric analysis. Over 48 h the biomass reached up to 0.49 ± 0.03 gPHA/gVSS (n=4). Samples were processed in parallel to characterise the distribution of...journal article 2022
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Hao, Xiaodi (author), Yu, W. (author), Yuan, Tugui (author), Wu, Yuanyuan (author), van Loosdrecht, Mark C.M. (author)As a product of phosphorous recovery from anaerobic digestion (AD) of waste activated sludge (WAS), vivianite has received increasing attention. However, key factors controlling vivianite formation have not yet been fully addressed. Thus, this study was initiated to ascertain key factors controlling vivianite formation. A simulation of...journal article 2022
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Pei, R. (author), Estevez Alonso, A. (author), Ortiz-Seco, Laura (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Municipal activated sludge can be used for polyhydroxyalkanoate (PHA) production, when supplied with volatile fatty acids. In this work, standardized PHA accumulation assays were performed with different activated sludge to determine (1) the maximum biomass PHA content, (2) the degree of enrichment (or volume-to-volume ratio of PHA...journal article 2022