Lane, R. (author), Vos, Y. (author), Wolters, A.E. (author), van Kessel, L.C.P.M. (author), Chen, S. Elisa (author), Liv, Nalan (author), Klumperman, Judith (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author) Large-scale electron microscopy (EM) allows analysis of both tissues and macromolecules in a semi-automated manner, but acquisition rate forms a bottleneck. We reasoned that a negative bias potential may be used to enhance signal collection, allowing shorter dwell times and thus increasing imaging speed. Negative bias potential has previously...
journal article 2021