Optogenetics and Light-Sheet Microscopy

Light-sheet microscopy is a powerful method for imaging small translucent samples in vivo, owing to its unique combination of fast imaging speeds, large field of view, and low phototoxicity. This chapter briefly reviews state-of-the-art technology for variations of light-sheet microscopy. We review recent examples of optogenetics in...

Optical field sampling for imaging and optical testing

This dissertation has mainly aimed at developing novel techniques, methodologies for measuring the optical field, specifically both the amplitude and phase distribution. Furthermore, we have attempted to extend their applications in the scope of optical imaging, including lensless/holographic imaging, quantitative phase imaging and the...

Light-sheet optimization for microscopy

Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is...