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Holtgrefe, Tim (author)Microtubules are long cylindrical polymers, assembled from tubulin proteins. Microtubule ends can be visualized using fluorescence and confocal microscopy. This allows for the study of microtubule dynamics. However, the manual annotation of microtubules is laborious, which is why automated tracking methods are used. In this project we have...bachelor thesis 2023
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Amini Hounejani, R. (author)The past decades have seen the rapid development of many aspects of synthetic biology. For example, attempts to build synthetic cells under controlled conditions in the laboratory have led to significant achievements. Following a bottom-up approach, scientists aim at building a self-reproducing synthetic cell with a minimum number of biological...doctoral thesis 2023
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Polley, Soumitra (author), Müschenborn, Helen (author), Terbeck, Melina (author), De Antoni, Anna (author), Vetter, Ingrid R. (author), Dogterom, A.M. (author), Musacchio, Andrea (author), Volkov, V. (author), Huis in 't Veld, Pim J. (author)During cell division, kinetochores link chromosomes to spindle microtubules. The Ndc80 complex, a crucial microtubule binder, populates each kinetochore with dozens of copies. Whether adjacent Ndc80 complexes cooperate to promote microtubule binding remains unclear. Here we demonstrate that the Ndc80 loop, a short sequence that interrupts the...journal article 2023
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Nick Maleki, A. (author), Huis In 't Veld, Pim J. (author), Akhmanova, Anna (author), Dogterom, A.M. (author), Volkov, V. (author)Microtubules are dynamic cytoskeletal filaments that can generate forces when polymerizing and depolymerizing. Proteins that follow growing or shortening microtubule ends and couple forces to cargo movement are important for a wide range of cellular processes. Quantifying these forces and the composition of protein complexes at dynamic...journal article 2023
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Ndiaye, A.B.E. (author), Koenderink, G.H. (author), Shemesh, M. (author)The mammalian cytoskeleton forms a mechanical continuum that spans across the cell, connecting the cell surface to the nucleus via transmembrane protein complexes in the plasma and nuclear membranes. It transmits extracellular forces to the cell interior, providing mechanical cues that influence cellular decisions, but also actively generates...review 2022
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Kuzmić, Mira (author), Castro Linares, G. (author), Leischner Fialová, Jindřiška (author), Iv, François (author), Salaün, Danièle (author), Llewellyn, Alex (author), Gomes, Maxime (author), Belhabib, Mayssa (author), Koenderink, G.H. (author)Septins, a family of GTP-binding proteins that assemble into higher order structures, interface with the membrane, actin filaments and microtubules, and are thus important regulators of cytoarchitecture. Septin 9 (SEPT9), which is frequently overexpressed in tumors and mutated in hereditary neuralgic amyotrophy (HNA), mediates the binding of...journal article 2022
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Alkemade, C. (author)The human body is composed of about 3−4×1013 (30-40 trillion) cells [1]. These cells are all functioning consistently, and working elegantly together, to sustain the organism. Not only humans, but all other living things on earth (from plants to parrots) are composed of cells. Cells are the smallest living building blocks of plants and animals,...doctoral thesis 2021
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Kattan, J.M. (author), Doerr, A. (author), Dogterom, A.M. (author), Danelon, C.J.A. (author)Genetic control over a cytoskeletal network inside lipid vesicles offers a potential route to controlled shape changes and DNA segregation in synthetic cell biology. Bacterial microtubules (bMTs) are protein filaments found in bacteria of the genus Prosthecobacter. They are formed by the tubulins BtubA and BtubB, which polymerize in the...journal article 2021
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Vendel, K.J.A. (author)Every living organism consists of cells. Even for the simplest single-cell organism, this cell is extremely complex. Thousands of components (such asDNA, cytoskeletal filaments, proteins, lipids, nutrients and energy) are organized both spatially and temporally to ensure proper functioning of vital cellular processes. One of those processes is...doctoral thesis 2020
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Kattan, J.M. (author)Humanity has achieved to decipher the most fundamental mechanics of cellular life. Nevertheless, despite intense efforts there are still considerable gaps in our understanding of cellular processes. Traditionally, biologists investigate life by observation of existing lifeforms. In order to assign functions to biological components, it is common...doctoral thesis 2020
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Kok, M.W.A. (author)The microtubule cytoskeleton is an intracellular polymer network involved in cell shape, cell motility, and cell division. Microtubules are self-assembling, dynamic polymers composed of tubulin proteins that alternate between phases of growth and shrinkage, a behaviour known as “dynamic instability”. A key feature of microtubules is their...doctoral thesis 2019
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Vendel, K.J.A. (author), Tschirpke, S. (author), Shamsi, F. (author), Dogterom, A.M. (author), Laan, L. (author)Cell polarity - the morphological and functional differentiation of cellular compartments in a directional manner - is required for processes such as orientation of cell division, directed cellular growth and motility. How the interplay of components within the complexity of a cell leads to cell polarity is still heavily debated. In this...review 2019
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Huis In 't Veld, Pim J. (author), Volkov, V. (author), Stender, Isabelle D. (author), Musacchio, Andrea (author), Dogterom, A.M. (author)Errorless chromosome segregation requires load-bearing attachments of the plus ends of spindle microtubules to chromosome structures named kinetochores. How these end-on kinetochore attachments are established following initial lateral contacts with the microtubule lattice is poorly understood. Two microtubule-binding complexes, the Ndc80 and...journal article 2019
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McHugh, Toni (author), Zou, Juan (author), Volkov, V. (author), Bertin, Aurélie (author), Talapatra, Sandeep K. (author), Rappsilber, Juri (author), Dogterom, A.M. (author), Welburn, Julie P.I. (author)Kinesin-13 motors regulate precise microtubule dynamics and limit microtubule length throughout metazoans by depolymerizing microtubule ends. Recently, the kinesin-13 motor family member MCAK (also known Kif2C) has been proposed to undergo large conformational changes during its catalytic cycle, as it switches from being in solution to being...journal article 2019
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Aher, Amol (author), Kok, M.W.A. (author), Sharma, Ashwani (author), Rai, Ankit (author), Olieric, Natacha (author), Rodriguez-Garcia, Ruddi (author), Katrukha, Eugene A. (author), Weinert, Tobias (author), Olieric, Vincent (author), Kapitein, Lukas C. (author), Steinmetz, Michel O. (author), Dogterom, A.M. (author), Akhmanova, Anna (author)The dynamic instability of microtubules plays a key role in controlling their organization and function, but the cellular mechanisms regulating this process are poorly understood. Here, we show that cytoplasmic linker-associated proteins (CLASPs) suppress transitions from microtubule growth to shortening, termed catastrophes, including those...journal article 2018
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Tas, Roderick P. (author), Chen, Chiung Yi (author), Katrukha, Eugene A. (author), Vleugel, M. (author), Kok, M.W.A. (author), Dogterom, A.M. (author), Akhmanova, Anna (author), Kapitein, Lukas C. (author)Force generation by molecular motors drives biological processes such as asymmetric cell division and cell migration. Microtubule gliding assays in which surface-immobilized motor proteins drive microtubule propulsion are widely used to study basic motor properties as well as the collective behavior of active self-organized systems....journal article 2018
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Taberner Carretero, N. (author)In this thesis we took the challenge to in vitro reconstitute a minimal phenomenon essential for live: Cell polarity. This is an ubiquitous phenomenon that allows cells to define a direction for migration, growth or division. Our study focussed on microtubulebased establishment of polarity taking S. pombe as a model organism. In this rod-shaped...doctoral thesis 2016
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Vleugel, M. (author), Kok, M.W.A. (author), Dogterom, A.M. (author)Microtubules switch between growing and shrinking states, a feature known as dynamic instability. The biochemical parameters underlying dynamic instability are modulated by a wide variety of microtubule-associated proteins that enable the strict control of microtubule dynamics in cells. The forces generated by controlled growth and shrinkage...journal article 2016
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Vleugel, M. (author), Roth, S.C.A. (author), Groenendijk, Celebrity F. (author), Dogterom, A.M. (author)Mitotic spindle assembly, positioning and orientation depend on the combined forces generated by microtubule dynamics, microtubule motor proteins and cross-linkers. Growing microtubules can generate pushing forces, while depolymerizing microtubules can convert the energy from microtubule shrinkage into pulling forces, when attached, for...journal article 2016
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Recouvreux, P. (author), Sokolowski, T.R. (author), Grammoustianou, A. (author), TenWolde, P.R. (author), Dogterom, A.M. (author)Cell polarity refers to a functional spatial organization of proteins that is crucial for the control of essential cellular processes such as growth and division. To establish polarity, cells rely on elaborate regulation networks that control the distribution of proteins at the cell membrane. In fission yeast cells, a microtubule-dependent...journal article 2016
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