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Hülsen, Tim (author), Venturato, Daniel (author), Chan, Clement (author), Vandi, Luigi (author), Laycock, Bronwyn (author), Pratt, Steven (author), Stegman, Samuel (author), van Loosdrecht, Mark C.M. (author), Batstone, Damien J. (author)Anoxygenic purple phototrophic bacteria (PPB) were utilised in an 80 L biofilm flat plate photobioreactor to generate polyhydroxyalkanoate (PHA) over 44 cycles, with acetate as feed. Over the cycles, net PHA yield (growth + accumulation) averaged 21% while accumulation yield averaged 55%. Average PHA content was 35 wt% volatile solids (VS),...journal article 2024
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Werker, A. (author), Pei, R. (author), Kim, Kevin (author), Moretto, Giulia (author), Estevez Alonso, A. (author), Vermeer, C.M. (author), Arcos-Hernandez, Monica (author), Dijkstra, Jelmer (author), de Vries, Erik (author)Past studies have repeatedly demonstrated the technical feasibility to produce polyhydroxyalkanoate (PHA) using bacterial biomass of mixed microbial cultures (MMCs). Commercial quality grades of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), PHBV, can be produced with control of average monomer composition. However, demonstration of PHBV...journal article 2023
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Roibas Rozas, A. (author), Saavedra del Oso, Mateo (author), Posada Duque, J.A. (author), Mosquera-Corral, Anuska (author), Hospido, Almudena (author)Mussels cooking wastewater (MCW) and fish processing wastewater (FPW) were utilized as feedstocks for the production of polyhydroxyalkanoates (PHA) and triacylglycerides (TAG) at the laboratory scale. This study presents a comparison of the techno-economic and environmental performance of ten circular economy-based and innovative processes,...journal article 2023
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Pei, R. (author), Vicente-Venegas, Gerard (author), Tomaszewska-Porada, Agnieszka (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Polyhydroxyalkanoates (PHAs) can be produced with municipal waste activated sludge from biological wastewater treatment processes. Methods of selective fluorescent staining with confocal laser scanning microscopy (CLSM) were developed and optimized to evaluate the distribution of PHA storage activity in this mixed culture activated sludge...journal article 2023
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Pei, R. (author), Tarek-Bahgat, N. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Poly(3-hydroxybutyrate) (PHB) was accumulated in full-scale municipal waste activated sludge at pilot scale. After accumulation, the fate of the PHB-rich biomass was evaluated over two weeks as a function of initial pH (5.5, 7.0 and 10), and incubation temperature (25, 37 and 55 °C), with or without aeration. PHB became consumed under aerobic...journal article 2023
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Rossi, E. (author), Raghavan, A. (author), Copuroglu, Oguzhan (author), Jonkers, H.M. (author)In this study, the applicability of two bacteria-based healing agents (e.g., poly-lactic acid and polyhydroxyalkanoate) in blast furnace slag cement (BFSC) mortar has been assessed. An experimental campaign on the functional properties, self-healing capacity, freezing–thawing and carbonation resistance has been conducted in comparison with plain...journal article 2022
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Estevez Alonso, A. (author), Arias-Buendía, María (author), Pei, R. (author), van Veelen, H. Pieter J. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Activated sludge from municipal wastewater treatment processes can be used directly for the production of biodegradable polyesters from the family of polyhydroxyalkanoates (PHAs). However, municipal activated sludge typically cannot accumulate PHAs to very high levels and often low yields of polymer produced on substrate are observed. In the...journal article 2022
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Pei, R. (author), Vicente-Venegas, Gerard (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Polyhydroxyalkanoate accumulation experiments at pilot scale were performed with fullscale municipal waste activated sludge. Development of biomass PHA content was quantified by thermogravimetric analysis. Over 48 h the biomass reached up to 0.49 ± 0.03 gPHA/gVSS (n=4). Samples were processed in parallel to characterise the distribution of...journal article 2022
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Pei, R. (author), Estevez Alonso, A. (author), Ortiz-Seco, Laura (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Municipal activated sludge can be used for polyhydroxyalkanoate (PHA) production, when supplied with volatile fatty acids. In this work, standardized PHA accumulation assays were performed with different activated sludge to determine (1) the maximum biomass PHA content, (2) the degree of enrichment (or volume-to-volume ratio of PHA...journal article 2022
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Vermeer, C.M. (author), Nielsen, Maaike (author), Eckhardt, Vincent (author), Hortensius, Matthijs (author), Tamis, J. (author), Picken, S.J. (author), Meesters, G.M.H. (author), Kleerebezem, R. (author)The biotechnological production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) derived from organic waste streams by mixed microbial communities is well established at the pilot-level. However, there is limited research on the recovery of the biopolymer from the microbial biomass, while its impact on product quality and product costs...journal article 2022
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Argiz, Lucía (author), Correa-Galeote, David (author), Val del Río, Ángeles (author), Mosquera-Corral, Anuska (author), Gonzalez Cabaleiro, R. (author)The lipid fraction of the effluents generated in several food-processing activities can be transformed into polyhydroxyalkanoates (PHAs) and triacylglycerides (TAGs), through open culture biotechnologies. Although competition between storing and non-storing populations in mixed microbial cultures (MMCs) has been widely studied, the right...journal article 2022
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Vermeer, C.M. (author), Bons, Larissa J. (author), Kleerebezem, R. (author)Abstract: Using microbial enrichment cultures for the production of waste-derived polyhydroxyalkanoates (PHAs) is a promising technology to recover secondary resources. Volatile fatty acids (VFAs) form the preferred substrate for PHA production. Isobutyrate is a VFA appearing in multiple waste valorization routes, such as anaerobic...journal article 2022
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Vermeer, C.M. (author), Depaz, Lena (author), van den Berg, Emily (author), Koelmans, Tom (author), Kleerebezem, R. (author)Medium-chain-length polyhydroxyalkanoate (mcl-PHA) production by using microbial enrichments is a promising but largely unexplored approach to obtain elastomeric biomaterials from secondary resources. In this study, several enrichment strategies were tested to select a community with a high mcl-PHA storage capacity when feeding octanoate. On...journal article 2022
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Estevez Alonso, A. (author), Altamira-Algarra, Beatriz (author), Arnau-Segarra, César (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)The developments of mixed culture polyhydroxyalkanoate production has been directed to maximize the biomass PHA content with limited attention to polymer quality. Direct comparison of PHA accumulation literature is challenging, and even regularly contradicting in reported results, due to underlying differences that are not well expressed. A...journal article 2022
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Stouten, G.R. (author)Quickly, the show is about the start. Date: 3.5 thousand million years ago, location: planet Earth, event: life. Naturally, life is starting small, even microscopically tiny. Life in the form of microorganisms endures eons of time in which the world changes. They survived, failed, adapted, thrived, and they actually changed the world. They have...doctoral thesis 2021
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Vermeer, C.M. (author), Rossi, E. (author), Tamis, J. (author), Jonkers, H.M. (author), Kleerebezem, R. (author)Polyhydroxyalkanoate (PHA) production is a promising opportunity to recover organic carbon from waste streams. However, widespread application of waste-derived PHA as biodegradable plastic is restricted by expensive purification steps, high quality requirements, and a fierce competition with the conventional plastic market. To overcome these...journal article 2021
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Salem, Rana (author), Soliman, Moomen (author), Fergala, A.M.A.A. (author), Audette, Gerald F. (author), Eldyasti, Ahmed (author)With the adverse environmental ramifications of the use of petroleum-based plastic out-weighing the challenges facing the industrialization of bioplastics, polyhydroxyalkanoate (PHA) biopolymer has gained broad interest in recent years. Thus, an efficient approach for maximizing polyhydroxybutyrate (PHB) polymer production in methanotrophic...journal article 2021
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Estevez Alonso, A. (author), Pei, R. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Conversion of organic waste and wastewater to polyhydroxyalkanoates (PHAs) offers a potential to recover valuable resources from organic waste. Microbial community-based PHA production systems have been successfully applied in the last decade at lab- and pilot-scales, with a total of 19 pilot installations reported in the scientific...review 2021
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Estevez Alonso, A. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Microbial community-based polyhydroxyalkanoate (PHA) production has been demonstrated repeatedly at pilot scale. Ammonium, normally present in waste streams, might be oxidized by nitrifying bacteria resulting in additional aeration energy demand. The use of low dissolved oxygen (DO) concentrations allowed to reduce nitrifying rates by up to...journal article 2021
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Mulders, M. (author), Tamis, J. (author), Stouten, G.R. (author), Kleerebezem, R. (author)The wide variety of organic carbon to nitrogen and phosphorous ratios that are encountered in different wastewaters has a major impact on the poly(3-hydroxybutyrate) (PHB) accumulation potential of microbial communities. In this study we investigated the influence of the substrate composition in terms of the carbon to nitrogen (C/N) or...journal article 2020
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