Shape anisotropic colloidal particle fabrication using 2-photon polymerization

Hypothesis: Our ability to dictate the colloid geometry is intimately related to self-assembly. The synthesis of anisotropic colloidal particles is currently dominated by wet chemistry and lithographic techniques. The wet chemical synthesis offers limited particle geometries at bulk quantities. Lithographic techniques, on the other hand,...

Polymer nano manufacturing of a biomimicking surface used for kidney stone crystallization studies

Kidney stone disease is an increasing worldwide issue. There is a current lack of understanding of the exact mechanisms involved, partially due to the need for experimental instrumentation able to mimic the microenvironmental conditions present in vivo. As crystal nucleation often initiates at liquid-solid interfaces, the interface morphology...

Submicron patterns-on-a-chip: Fabrication of a microfluidic device incorporating 3D printed surface ornaments

Manufacturing high throughput in vitro models resembling the tissue microenvironment is highly demanded for studying bone regeneration. Tissues such as bone have complex multiscale architectures inside which cells reside. To this end, engineering a microfluidic platform incorporated with three-dimensional (3D) microscaffolds and submicron...

3D-microfabrication by two-photon polymerization of an integrated sacrificial stencil mask

This work aims at developing a new and unconventional Sacrificial Stencil Mask (SSM) technology by exploiting Two-Photon Polymerization (2PP) in an IP-L/SU-8 double layer resist system. The process consists of the sequential deposition of two different resists, such as SU-8 and IPL, onto the same glass substrate, followed by 2PP lithography...

Multiscale 3D-printing of a suspended hollow microfluidic device

Suspended hollow micro-cantilevers have been employed for zeptogram (10^-21 g) mass measurements, pipetting of femtoliter<br/>(10^-15 L) volumes, and studies on mechanical interaction with single cells. The main fabrication method of these devices is by standard clean room micro/nano fabrication techniques, however, they are inherently planar,...

Developing a microfluidic device incorporating submicron topographies for studying bone regeneration

A major challenge facing the study of bone regeneration today is the inability to mimic the tissue microenvironment and the factors comprising it. Organ-on-chip based microfluidic systems today aim at doing so by dynamically culturing living cells in them. These are preferred owing to their small feature sizes, laminar flows, high throughput,...

Manufacturing of a polymeric membrane with integrated porous electrode

A manufacturing technology is proposed to manufacture polymeric membrane scaffolds for culturing of cells, tissues, and organoids with integrated sensor capabilities and fluidic functionalities. A multilayer substrate is imprinted with a soft mold to manufacture polymeric membrane scaffolds for culturing of cells, tissues, and organoids with...

Mass and stiffness measurement using a multi-modal analysis

Biomechanics of cells have been identified as an important factor relating to their functionalities. Consequently, the need for sensitive measurement methods for mechanical analysis on the nano- and microscale is high. Micro-cantilever resonators can have a large impact in material determination at the nanoscale. When adding a material, the...