This study investigates the effects of warm-mix asphalt (WMA) additives and bio-oil on the rheological and chemical properties of virgin bitumen (VB) and polymer-modified bitumen (PMB) under varying aging conditions. The workability, viscoelasticity, and chemical characteristic of warm-mix bio-rejuvenated bitumen are assessed using a rotational viscometer, dynamic shear rheometer, Fourier Transform Infrared Spectroscopy. Results show that PMB has superior aging resistance than VB. The wax-based additive exponentially reduces viscosity of VB, while the chemical-based additive decreases viscosity linearly and performs better in PMB due to improved polymer-bitumen interfacial lubrication. The wax-based additive enhances high-temperature elasticity and rutting resistance, whereas adding 0.9 wt% chemical-based additive declines the rutting failure temperature (RFT) of VB by 3.3°C and PMB by 2.3°C. However, the wax-based additive lowers the fatigue life of VB, while the chemical-based additive extends the fatigue life. The fatigue failure temperature (FFT) value increases by 2.3°C for VB and 3.4°C for PMB after adding 4 % wax-based additive. The optimal dosage of the chemical-based additive for PMB is determined to be 0.6 %. The bio-rejuvenator significantly enhances the fatigue performance of aged VB, but has limited impact on aged PMB. Both WMA additives reduce aromaticity and alter aliphatic content, with the chemical-based one showing a stronger dilutive effect, particularly in PMB. Additionally, a warm-mix bio-rejuvenated bitumen with higher aliphatic index (AII) and carbonyl index (CI) shows better deformation resistance and longer fatigue life.