The ability to identify individual protein molecules using Surface-Enhanced Raman Scattering (SERS) spectroscopy, without the need for labelling, is a significant advancement in biomedical diagnostics. However, the inherently small Raman scattering cross-section of most (bio) molecules necessitates significant signal amplification for successful detection, particularly at the single-molecule level. A novel approach is introduced for fabricating plasmonic nanopores suitable for sequential Raman readout, namely the recording of Raman spectra from portions of molecules, which are progressively flowing into plasmonic hot spots. The method is based on Capillary-Assisted Particle Assembly (CAPA)of gold nanoparticles (Au NPs), thus ensuring high stability and cost-effectiveness. By electrophoretically driving polypeptides through these nanopores, real-time Raman detection is achieved with a Single-Photon Avalanche Diode (SPAD) camera, attaining single-molecule detection at 1 nm concentration with 100 microsecond resolution. Statistical analysis of translocation times, photon scattering rates, and spectral data confirms a linear correlation between dwell time, molecular length, and Raman signal intensity. On average, a photon scattering of 6 photons/amino acid and a translocation time of 7 µs per amino acid is recorded. These results demonstrate the feasibility of sequential Raman readout, overcoming key limitations of SERS. This method represents a significant step toward label-free, high-resolution molecular identification, with future potential applications in protein identification.

This article examines how a firm's willingness to make trade-offs that favour sustainability over commercial goals attenuates the relationship between firm-level sustainability orientation and subsequent performance. The hypothesis development draws on stakeholder theory and the literature on mission and revenue drifts, while the empirical analysis is based on two waves of original survey data on Finnish manufacturing SMEs. We find that sustainability orientation is positively associated with performance only when the willingness to make sustainability trade-offs is low, whereas the relationship becomes negative when the willingness to make such trade-offs is high. Our findings thus suggest that the popular adage of doing well by doing good might only hold if doing good does not conflict with business interests. The results add to stakeholder theory by showing how conforming to stakeholder expectations can be good for business – but only if doing so does not seriously compromise the pursuit of profits.