Introduction: Collaboration in teams composed of both humans and automation has an interdependent nature, which demands calibrated trust among all the team members. For building suitable autonomous teammates, we need to study how trust and trustworthiness function in such teams. In particular, automation occasionally fails to do its job, which leads to a decrease in a human’s trust. Research has found interesting effects of such a reduction of trust on the human’s trustworthiness, i.e., human characteristics that make them more or less reliable. This paper investigates how automation failure in a human-automation collaborative scenario affects the human’s trust in the automation, as well as a human’s trustworthiness towards the automation.Methods: We present a 2 × 2 mixed design experiment in which the participants perform a simulated task in a 2D grid-world, collaborating with an automation in a “moving-out” scenario. During the experiment, we measure the participants’ trustworthiness, trust, and liking regarding the automation, both subjectively and objectively.Results: Our results show that automation failure negatively affects the human’s trustworthiness, as well as their trust in and liking of the automation.Discussion: Learning the effects of automation failure in trust and trustworthiness can contribute to a better understanding of the nature and dynamics of trust in these teams and improving human-automation teamwork.