This work explores the dynamic response of a turbulent boundary layer to large-scale reactive opposition control, at a friction Reynolds number of Reτ≈2240. A surface-mounted hot-film is employed as the input sensor, capturing large-scale fluctuations in the wall-shear stress, and actuation is performed with a single on/off wall-normal blowing jet positioned 2.4δ downstream of the input sensor, operating with an exit velocity of vj=0.4U∞. Our study builds upon the work of Abbassi et al. [Int. J. Heat Fluid Flow 67, 30 (2017)0142727X10.1016/j.ijheatfluidflow.2017.05.003] and includes a control-calibration experiment and a performance assessment using PIV- and PTV-based flow field analyses. With the control-off calibration-experiment conducted a priori, a transfer kernel is identified so that the velocity fluctuations that are to-be-controlled can be estimated. The controller targets large-scale high-speed zones in an "opposing"mode and low-speed zones in a "reinforcing"mode. A desynchronized mode was tested for reference and consisted of a statistically similar control mode, but without synchronization to the incoming velocity fluctuations. An energy-attenuation of about 40 % is observed for the opposing control mode in the frequency band corresponding to the passage of large-scale motions. This proves the effectiveness of the control in targeting large-scale motions: an energy-intensification of roughly 45% occurs for the reinforcing control mode instead, while no change in energy, within the wall-normal range targeted, appears with the desynchronized control mode. Moreover, direct measures of the skin-friction drag are inferred from PTV data. Results indicate that the opposing control logic yields the lowest wall-shear stress (3% lower than the desynchronized control, and 10% lower than the uncontrolled flow). Finally, a FIK-decomposition of the skin-friction coefficient revealed that the off-the-wall turbulence follows a consistent trend with the PTV-based wall-shear stress measurements, although biased by an increased shear in the wake of the boundary layer given the formation of a plume due to the jet-in-crossflow actuation.