In the last decades of the twentieth century, a shift from the industrial economy towards a more knowledge-based economy was made. Due to the economic crisis the Zuidvleugel invented three economic pillars. The knowledge axis is one of them. This graduation project researched the position of Leiden in this knowledge axis. To improve the knowledge location of Leiden, a new masterplan has been made. This masterplan should stimulate interaction between different users of this knowledge location. The main question for the graduation project is: In what way can a new masterplan help develop the knowledge cluster of Leiden towards the main innovative life science cluster?